Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.8K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.8K
Replication in Eukaryotes01:29

Replication in Eukaryotes

18.3K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.3K
Replication in Eukaryotes02:31

Replication in Eukaryotes

206.6K
Overview
206.6K
The DNA Replication Fork01:02

The DNA Replication Fork

42.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
42.1K
The DNA Replication Fork01:02

The DNA Replication Fork

19.0K
19.0K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Cellular replisomes are powered by flex-fuel motors for unwinding DNA.

Nature communications·2026
Same author

A modular framework for automated segmentation and analysis of AFM imaging of chromatin organization.

Nucleic acids research·2026
Same author

Structural and cellular insights into DCTPP1 antagonists and their synergistic action with DNMT inhibitors.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Supercoiled DNA recognition and cleavage control in topoisomerase VI.

Nature communications·2026
Same author

Chromatin buffers torsional stress during transcription.

Science (New York, N.Y.)·2025
Same author

AlphaFold-guided phylogenetic analyses suggest surprising heterogeneity in metazoan replication origin licensing mechanisms.

The EMBO journal·2025
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Mar 7, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.7K

Mecanismos para iniciar la replicación del ADN celular

Franziska Bleichert1, Michael R Botchan2, James M Berger1

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. franziska.bleichert@fmi.ch mbotchan@berkeley.edu jmberger@jhmi.edu.

Science (New York, N.Y.)
|February 18, 2017
PubMed
Resumen
Este resumen es generado por máquina.

La replicación del ADN se basa en las helicasas, cargadas por proteínas iniciadoras y cargadoras. Este estudio compara cómo estas proteínas depositan las helicasas y desenrollan el ADN a través de la vida

Más Videos Relacionados

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.6K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Videos de Experimentos Relacionados

Last Updated: Mar 7, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.7K
Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.6K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Área de la Ciencia:

  • Biología molecular
  • La genética
  • La bioquímica

Sus antecedentes:

  • La replicación celular del ADN requiere helicasas hexámicas en forma de anillo para desenrollar la hélice del ADN.
  • Las helicasas replicativas se cargan en el ADN mediante proteínas iniciadoras, cargadoras y accesorias durante la iniciación de la replicación.
  • Este proceso está estrictamente regulado e implica múltiples pasos.

Objetivo del estudio:

  • Para discutir la coreografía molecular de la iniciación de la replicación del ADN en los tres dominios de la vida.
  • Para resaltar las similitudes y diferencias en la deposición de helicasa y las estrategias de desenrollo del ADN.
  • Para entender la divergencia evolutiva de las proteínas iniciadoras y cargadoras.

Principales métodos:

  • Análisis comparativo de los mecanismos de iniciación de la replicación del ADN.
  • Revisión de la coreografía molecular y las interacciones de proteínas.
  • Comparación filogenética de las proteínas iniciadoras y cargadoras.

Principales resultados:

  • Existen similitudes y diferencias en la deposición de helicasas y las estrategias de desenrollo del ADN en bacterias, arqueas y eucariotas.
  • Las proteínas iniciadoras y cargadoras, a pesar de la relación filogenética, muestran una divergencia considerable e impredecible en sus mecanismos.
  • La regulación de la iniciación de la replicación del ADN se conserva pero es mecánicamente diversa.

Conclusiones:

  • El proceso fundamental de iniciación de la replicación del ADN se conserva a lo largo de la vida.
  • La evolución ha llevado a diversos mecanismos moleculares para la carga de la helicasa y el desenrollo del ADN.
  • La comprensión de estas estrategias divergentes proporciona una visión de la evolución de la maquinaria de replicación del ADN.