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A Protocol for the Production of KLRG1 Tetramer
Published on: January 13, 2010
Los leucocitos expresan un nuevo gen que codifica una putativa proteína-cinasa transmembrana desprovista de un
1Lautenberg Center for General & Tumor Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Nature
|June 16, 1988
Resumen
Los investigadores descubrieron un nuevo gen leucocitario de tirosina quinasa (ltk), expresado principalmente en leucocitos. Esta proteína transmembrana, que carece de un dominio extracelular, puede funcionar como una subunidad de transducción de señales para los receptores hematopoyéticos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La señalización celular de las células.
- Inmunología Inmunología.
Sus antecedentes:
- Las kinasas de proteína-tirosina controlan el crecimiento y la diferenciación celular.
- La mayoría de las tirosinacinasas conocidas están ampliamente expresadas, a diferencia de las específicas de los tejidos.
- Las linfoquinas pueden regular la supervivencia de las células hematopoyéticas a través del transporte de glucosa y la actividad de los receptores de insulina.
Objetivo del estudio:
- Investigar la expresión de genes similares a los receptores de insulina en las células hematopoyéticas.
- Aislar y caracterizar un nuevo gen potencialmente involucrado en la señalización de leucocitos.
Principales métodos:
- Utilizó el oncogén v-ros como una sonda para detectar genes relacionados.
- Aislaron y caracterizaron el ADN complementario del gen ltk.
- Se utilizaron anticuerpos para identificar algunas proteínas en el timo de un ratón.
Principales resultados:
- Se identificó un nuevo gen, ltk (leucócito tirosina quinasa), expresado principalmente en leucocitos.
- Está relacionado con la familia de las receptoras de insulina tirosina quinasa, pero carece de un dominio extracelular.
- Se identificaron dos proteínas ltk candidatas como proteínas de membrana integral en el timo del ratón.
Conclusiones:
- Es una nueva tirosina quinasa específica para leucocitos.
- Su estructura única sugiere un papel como una subunidad de transducción de señales para los receptores hematopoyéticos.
- Este hallazgo abre nuevas vías para la comprensión de las vías de señalización de las células inmunes.
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