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Asociación entre una secuencia de ADN satelital y el Responder de Distorsión de Segregación en D. melanogaster

C I Wu¹, T W Lyttle, M L Wu

¹Department of Biology, University of Rochester, New York 14627.

|July 15, 1988

Resumen

Se ha planteado la hipótesis de que las repeticiones de ADN satélite son el elemento Responder (Rsp) en D. melanogaster, que causa disfunción de los espermatozoides y conducción meiotica. Esta matriz de repetición de la matriz.

Área de la Ciencia:

Genética La genética.
Biología Molecular Biología Molecular
Biología evolutiva Biología evolutiva.

Sus antecedentes:

El sistema genético del Disturbante de Segregación (SD) en Drosophila melanogaster influye en el impulso meiótico.
El elemento Responder (Rsp) es un componente clave de SD, asociado con las secuencias de ADN satelitales.
Rsp causa la condensación aberrante de la cromatina durante la espermiogénesis, lo que lleva a una reducción de la transmisión de los espermatozoides portadores de Rsp.

Objetivo del estudio:

Para investigar la identidad molecular del elemento Responder (Rsp).
Determinar el papel de las secuencias de ADN satelitales asociadas en la función de Rsp y la unidad meiótica.
Comprender la dinámica estructural y la plasticidad evolutiva del locus Rsp.

Principales métodos:

Análisis de las matrices de ADN satelitales asociadas con Rsp.
Examen de las deleciones y translocaciones de Rsp en el cromosoma Y.
Correlación del número de copias repetidas con la sensibilidad de la unidad meiotica.

Principales resultados:

Una gran variedad de secuencias de ADN satelitales se encuentra consistentemente con el elemento Rsp.
La matriz de repetición de satélite se elimina o se transloca junto con Rsp.
El número de repeticiones de satélite se correlaciona fuertemente con la sensibilidad a la unidad meiótica.

Conclusiones:

Se propone que la matriz de repetición de ADN satelital constituya el elemento Responder (Rsp) funcional.
La variabilidad y la dinámica estructural del locus sugieren una evolución molecular activa.
Comprender el locus Rsp proporciona información sobre la evolución de la unidad meiótica y los elementos repetitivos del ADN.

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