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Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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Los eventos de inversión en el genoma del HSV-1 están directamente mediados por la maquinaria de replicación del ADN
P C Weber1, M D Challberg, N J Nelson
1Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.
Cell
|July 29, 1988
Resumen
Se observó una inversión de secuencia de alta frecuencia del elemento transponible bacteriano Tn5 en los genomas del virus del herpes simple tipo 1 (HSV-1). Esta inversión está mediada por los genes de replicación del ADN del HSV-1, no por la recombinación específica del sitio.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- El elemento transponible bacteriano Tn5 exhibe una inversión de secuencia.
- El virus del herpes simple tipo 1 (HSV-1) sufre un reordenamiento del genoma durante la infección.
Objetivo del estudio:
- Para investigar el mecanismo de la inversión de la secuencia Tn5 dentro del genoma HSV-1.
- Para determinar si la replicación del ADN del HSV-1 está involucrada en la inversión de Tn5.
Principales métodos:
- Análisis de supresión de los elementos IS50 en Tn5.5.
- Evaluación del papel de los genes de replicación del ADN del HSV-1 en la mediación de la inversión de Tn5.
- Analizando la inversión de las secuencias duplicadas de unión L-S del genoma HSV-1.
Principales resultados:
- La inversión de la secuencia Tn5 ocurrió con alta frecuencia en los genomas del HSV-1.
- El análisis de eliminación no reveló señales de acción cis para la inversión dentro de los elementos IS50.
- Siete genes esenciales de síntesis de ADN HSV-1 mediaron la inversión de Tn5 independientemente de otras funciones virales.
- Se observaron patrones de inversión similares para las secuencias de unión L-S duplicadas.
Conclusiones:
- La inversión de Tn5 en el HSV-1 no es específica del sitio.
- El proceso es un evento de recombinación generalizada.
- La maquinaria de replicación del ADN del HSV-1, no las secuencias virales específicas, impulsa la inversión del genoma.
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