Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Intersegmental transfers drive target search in an RNA-targeting CRISPR system.

bioRxiv : the preprint server for biology·2026
Same author

Pooled combinatorial screening identifies transcription factor sets that drive hematopoietic progenitor-like cell fate.

Cell systems·2026
Same author

Deep learning-enabled discovery of antibiotics effective against <i>Neisseria gonorrhoeae</i>.

Science translational medicine·2026
Same author

Aging disrupts spatiotemporal coordination in the cycling murine ovary.

Nature aging·2026
Same author

Rapid development and field evaluation of a portable CRISPR-based assay for Mpox during the 2025 Sierra Leone outbreak.

Nature communications·2026
Same author

Multimodal control of Cas13d activity through domain insertion at an allosteric hotspot.

Nature communications·2026

Video Experimental Relacionado

Updated: Jun 15, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Detección de ácido nucleico con CRISPR-Cas13a/C2c2

Jonathan S Gootenberg1,2,3,4,5, Omar O Abudayyeh1,2,3,4,6, Jeong Wook Lee7

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|April 15, 2017
PubMed
Resumen

Un nuevo diagnóstico basado en CRISPR (CRISPR-Dx) llamado SHERLOCK ofrece una detección rápida y altamente sensible de ácido nucleico. Esta herramienta molecular puede identificar virus específicos, bacterias y mutaciones, incluso en entornos de campo.

Más Videos Relacionados

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Videos de Experimentos Relacionados

Last Updated: Jun 15, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Área de la Ciencia:

  • Biología molecular
  • Biotecnología
  • La genética

Sus antecedentes:

  • La detección de ácido nucleico es crucial para el diagnóstico, la identificación de patógenos y el monitoreo de enfermedades.
  • El sistema CRISPR-Cas13a ofrece capacidades de orientación de ARN con actividad enzimática colateral.
  • Los métodos existentes pueden carecer de la velocidad, sensibilidad o especificidad requeridas para aplicaciones en el punto de atención.

Objetivo del estudio:

  • Desarrollar una plataforma de detección de ácido nucleico rápida, barata y altamente sensible.
  • Para aprovechar la actividad colateral de Cas13a con fines de diagnóstico.
  • Crear una herramienta versátil para la detección de patógenos, genotipo y identificación de mutaciones.

Principales métodos:

  • La actividad colateral de la ribonucleasa de Cas13a combinada con la amplificación isotérmica.
  • Desarrolló una plataforma de diagnóstico basada en CRISPR llamada SHERLOCK (desbloqueo de reportero enzimático de alta sensibilidad específica).
  • Utilizó SHERLOCK para detectar cepas virales específicas (Zika, Dengue), bacterias, ADN humano y mutaciones de ADN tumoral libre de células.

Principales resultados:

  • Se ha obtenido sensibilidad atomolar y especificidad de desajuste de una sola base en la detección de ácido nucleico.
  • Demostró la capacidad de SHERLOCK para detectar varios objetivos incluyendo virus, bacterias y ADN humano.
  • Demostró el potencial de los reactivos de liofilización para la independencia de la cadena de frío y el despliegue de campo en papel.

Conclusiones:

  • SHERLOCK proporciona una poderosa herramienta de diagnóstico basada en CRISPR para la detección molecular rápida y sensible.
  • La versatilidad de la plataforma se extiende a la identificación de patógenos, el análisis genético y la detección de mutaciones de cáncer.
  • La liofilización y la reconstitución en papel permiten a SHERLOCK aplicaciones accesibles en el punto de atención y en el campo.