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Una proteína naturalmente inmunogénica asociada al virión específica para el VIH-2 y el SIV
1Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.
Nature
|September 15, 1988
Resumen
El marco de lectura abierto-x (orf-x) en el VIH-2 y el SIV no lo es en el VIH-1. Su producto genético es indispensable para la replicación in vitro, ayudando a distinguir el VIH-2 de la infección por VIH-1.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Inmunología Inmunología.
Sus antecedentes:
- El virus de inmunodeficiencia humana tipo 1 (VIH-1), el VIH tipo 2 (VIH-2), y los virus de inmunodeficiencia simio (SIV) comparten similitudes genómicas.
- Un marco de lectura abierto, orf-x, está presente en el VIH-2 y el SIV, pero ausente en el VIH-1.
- La conservación de la secuencia en orf-x sugiere un papel potencial en la infectividad o replicación del virus.
Objetivo del estudio:
- Para investigar el papel de orf-x en la replicación del SIV y el VIH-2.
- Para determinar si los productos orf-x están presentes en los viriones.
- Evaluar el potencial diagnóstico de orf-x y sus anticuerpos para diferenciar el VIH-2 de las infecciones por VIH-1.
Principales métodos:
- Introducción de codones de parada prematura en el gen orf-x.
- Ensayos de infectividad y replicación in vitro.
- Pruebas de detección de anticuerpos utilizando muestras de suero VIH-2 y SIV positivas y suero de mono seropositivo de SIV.
Principales resultados:
- Se encontró que los productos orf-x de SIVMAC y el VIH-2SBL-6669 estaban asociados con los viriones.
- La interrupción de orf-x con un codón de parada no afectó la infectividad o la replicación del virus in vitro.
- Se detectaron anticuerpos contra el producto orf-x en una proporción significativa de muestras positivas para el VIH-2 y el SIV, pero no en muestras o controles positivos para el VIH-1.
Conclusiones:
- El gen orf-x es prescindible para la replicación in vitro de SIVMAC.
- El producto del gen orf-x del VIH-2 o los anticuerpos contra él pueden servir como marcadores para distinguir el VIH-2 de la infección por VIH-1.
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