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Los sistemas reguladores modificados modulan la expresión génica de los comensales humanos en el intestino
Bentley Lim1, Michael Zimmermann1, Natasha A Barry1
1Department of Microbial Pathogenesis and Microbial Sciences Institute, Yale University School of Medicine, New Haven, CT 06536-0812, USA.
Cell
|April 22, 2017
Resumen
Los investigadores desarrollaron un nuevo sistema de expresión génica inducible para las bacterias Bacteroides. Esta herramienta permite un control preciso de la actividad génica en el intestino, lo que permite nuevos estudios sobre las interacciones entre el microbioma y el huésped.
Área de la Ciencia:
- Microbiología
- Biología molecular
- Biología de sistemas
Sus antecedentes:
- La microbiota intestinal juega un papel crucial en la salud y la enfermedad.
- Las herramientas genéticas limitadas para los anaerobios intestinales dificultan la investigación de las funciones del microbioma.
- Los promotores inducibles son esenciales para el análisis en tiempo real de las contribuciones de genes microbianos.
Objetivo del estudio:
- Desarrollar un sistema de expresión génica sintonizable para el prominente género bacteriano intestinal Bacteroides.
- Para permitir la modulación dinámica de la expresión génica de Bacteroides dentro del entorno intestinal.
- Investigar la relación entre las enzimas microbianas y las interacciones huésped-patógeno.
Principales métodos:
- Diseñó una plataforma de expresión controlada por inductores sintéticos para especies de Bacteroides.
- Se ha demostrado una represión completa en ausencia de inductor y una inducción rápida (4-5 órdenes de magnitud) tras la adición.
- Se administra el inductor a través del agua potable para modular la expresión génica en el intestino del ratón.
Principales resultados:
- Se estableció con éxito un sistema de expresión génica sintonizable para Bacteroides in vivo.
- Mostró la capacidad de controlar la expresión génica dinámicamente dentro del intestino.
- Utilizó el sistema para estudiar el impacto de la actividad comensal de la sialidasa en la liberación de ácido siálico.
Conclusiones:
- El sistema de expresión inducible desarrollado proporciona una herramienta poderosa para el estudio de Bacteroides en el intestino.
- Esta plataforma facilita el análisis en tiempo real de las funciones genéticas del microbioma y su impacto en la fisiología y la enfermedad del huésped.
- Permite nuevas vías para explorar la dinámica huésped-microbio-patógeno.
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