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Transformación oncogénica dependiente de glucocorticoides por tipo 16, pero no por tipo 11 Virus del papiloma humano
M M Pater1, G A Hughes, D E Hyslop
1Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Nature
|October 27, 1988
Resumen
El ADN del virus del papiloma humano (VPH) tipo 16, combinado con el oncogén Ha-ras y la dexametasona, puede transformar oncogénicamente las células primarias. Esto sugiere un papel de las hormonas en el desarrollo del cáncer cervical relacionado con el VPH.
Área de la Ciencia:
- Oncología Oncología.
- Virología Virología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El carcinoma de células escamosas cervicales es un cáncer común en las mujeres, fuertemente correlacionado con la infección por el virus del papiloma humano (VPH).
- El ADN integrado de los tipos 16 y 18 del VPH se encuentra en la mayoría de los tumores cervicales.
- Se sospecha que las hormonas y el tabaco son cofactores en la neoplasia cervical, con un elemento sensible a los glucocorticoides identificado en el VPH-16.
Objetivo del estudio:
- Investigar el potencial de transformación oncogénica del ADN del VPH-16 en combinación con el oncogén activado Ha-ras.
- Determinar el papel de la hormona glucocorticoide dexametasona en este proceso de transformación.
Principales métodos:
- Las células primarias fueron transfectadas con ADN HPV-16 y un oncogén Ha-ras humano activado.
- Se evaluó el efecto de la dexametasona en la transformación oncogénica.
Principales resultados:
- La transformación oncogénica se observó cuando las células primarias fueron expuestas al ADN HPV-16, Ha-ras activado y dexametasona simultáneamente.
- El ADN del VPH-11 no indujo la transformación en las mismas condiciones, lo que indica la especificidad del tipo de VPH.
- La dexametasona fue esencial para la transformación oncogénica observada.
Conclusiones:
- La combinación de ADN HPV-16, Ha-ras activado y dexametasona puede inducir la transformación oncogénica de las células primarias.
- Las hormonas glucocorticoides pueden actuar como cofactores cruciales en el desarrollo del cáncer cervical asociado al VPH.
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