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La formación de estructuras de replicación del ADN en las células infectadas por el virus del herpes requiere una
1Program on Cell and Developmental Biology, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|December 2, 1988
Resumen
La infección por el virus del herpes simple reubica los sitios de replicación del ADN celular en las estructuras virales. La proteína funcional ICP8 es esencial para esta redistribución, organizando las proteínas de replicación del ADN dentro del núcleo.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- Biología celular Biología celular.
Sus antecedentes:
- La síntesis del ADN eucariótico ocurre en complejos multienzimales en sitios nucleares discretos.
- La organización espacial de la replicación del ADN es crucial para la estabilidad del genoma.
Objetivo del estudio:
- Para investigar el impacto de la infección por el virus del herpes simple (VHS) en los sitios de replicación del ADN de las células huésped.
- Para determinar el papel de la proteína de replicación del ADN del HSV ICP8 en este proceso.
Principales métodos:
- Etiquetado de bromodeoxyuridina (BrdU) para identificar los sitios de replicación del ADN celular.
- Infección de las células con cepas de HSV de tipo salvaje y mutantes.
- Microscopía de inmunofluorescencia para visualizar el ICP8 y los sitios de replicación del ADN.
Principales resultados:
- La infección por HSV hace que los sitios de replicación del ADN celular se trasladen a las estructuras pre-replicativas virales.
- Estas estructuras virales contienen la proteína de replicación del ADN del HSV ICP8.8.
- Las cepas mutantes de HSV que carecen de ICP8 funcional no alteran la ubicación de los sitios de replicación del ADN de la célula huésped.
Conclusiones:
- La maquinaria de replicación del ADN celular se recluta en los sitios prereplicativos virales durante la infección por HSV.
- Se requiere un ICP8 funcional para la redistribución de los complejos de replicación del ADN celular.
- Una proteína viral específica puede organizar las proteínas de replicación del ADN del huésped dentro del núcleo.
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