Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.2K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.2K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.8K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.8K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

25.1K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.1K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.8K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.8K
Epigenetic Regulation01:37

Epigenetic Regulation

4.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.0K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Concurrent administration of BCMA and GPRC5D chimeric antigen receptor (CAR) T cells in advanced multiple myeloma.

Blood·2026
Same author

Deep dynamical models of single-cell multiomic velocities predict loss-of-function and rescue perturbations in B cells.

bioRxiv : the preprint server for biology·2026
Same author

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same author

<i>SPP1</i><sup>hi</sup> macrophages in fibrin niches promote hyperplastic tissue remodeling in rheumatoid arthritis synovium.

Science translational medicine·2026
Same author

Interleukin-12 induces rapid STAT4/DDX5-dependent remodeling of RNA polymerase II occupancy in NK cells.

The Journal of experimental medicine·2026
Same author

Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformation.

Developmental cell·2026

Video Experimental Relacionado

Updated: Mar 2, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

10.9K

Los estados de cromatina definen la disfunción y reprogramación de las células T específicas del tumor

Mary Philip1, Lauren Fairchild2,3, Liping Sun4

  • 1Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA.

Nature
|May 18, 2017
PubMed
Resumen
Este resumen es generado por máquina.

Las células T CD8 que se infiltran en el tumor existen en dos estados: uno plástico y reprogramable, el otro fijo y resistente. La identificación de marcadores de superficie predice qué células T disfuncionales pueden ser reprogramadas terapéuticamente para la inmunoterapia contra el cáncer.

Más Videos Relacionados

Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines
11:39

Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines

Published on: June 17, 2017

18.9K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

13.1K

Videos de Experimentos Relacionados

Last Updated: Mar 2, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

10.9K
Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines
11:39

Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines

Published on: June 17, 2017

18.9K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

13.1K

Área de la Ciencia:

  • Inmunología
  • Biología del cáncer
  • La epigenética

Sus antecedentes:

  • Las células T CD8 específicas del tumor en tumores sólidos a menudo se vuelven disfuncionales, lo que dificulta las respuestas inmunes antitumorales.
  • Los mecanismos epigenéticos que rigen la disfunción de las células T y su susceptibilidad a las terapias de reprogramación, como el bloqueo del punto de control inmune, siguen siendo poco conocidos.

Objetivo del estudio:

  • Investigar la regulación epigenética de la disfunción de las células T CD8 en los tumores.
  • Identificar estados distintos de células T dentro de los tumores y su potencial para la reprogramación terapéutica.
  • Descubrir marcadores de superficie que predicen la reprogramabilidad de las células T para la inmunoterapia del cáncer.

Principales métodos:

  • Análisis del estado de la cromatina en las células T CD8 que se infiltran en los tumores de ratón.
  • Identificación de los marcadores de superficie que distinguen las subpoblaciones de células T.
  • Comparación de las células T CD8 que se infiltran en los tumores de ratones y humanos.

Principales resultados:

  • Las células T CD8 en tumores de ratón se diferencian en dos estados discretos de cromatina: un estado disfuncional plástico y reprogramable y un estado disfuncional fijo y resistente.
  • Se identificaron marcadores de superficie específicos que diferencian las células T CD8 reprogramables de las no reprogramables.
  • Estos marcadores de superficie identificados también están presentes en las células T CD8 humanas que se infiltran en el tumor.

Conclusiones:

  • Los programas epigenéticos dictan distintos estados disfuncionales de células T CD8 dentro de los tumores, lo que influye en su potencial terapéutico.
  • Los marcadores de superficie pueden predecir la reprogramabilidad de las células T CD8 disfuncionales, ofreciendo un biomarcador para la inmunoterapia del cáncer.
  • La comprensión de estos perfiles epigenéticos y de marcadores de superficie es crucial para el desarrollo de inmunoterapias efectivas.