Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.8K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

10.9K
10.9K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

19.2K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

4.0K
4.0K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Multimodal Distillation and Fusion for Enhanced Age-Related Macular Degeneration Classification.

IEEE journal of biomedical and health informatics·2026
Same author

Degradation of tris-(2-chloroisopropyl) phosphate initiated by OH and ClO radicals in environment: Mechanisms, kinetics, toxicity and molecular design of substitutes.

Journal of environmental sciences (China)·2026
Same author

From mechanism to molecule: Atmospheric and aqueous degradation kinetics, toxicity assessment, and sustainable redesign of the flame retardant TDCPP.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Hangover regulates gene expression by limiting NSL-mediated H4K16 acetylation.

Nucleic acids research·2025
Same author

CMOS: Confidence-Guided Multi-Scale Semi-Supervised Segmentation for Retinal Layers in OCT Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Histone modification cross-talk and protein complex diversification confer plasticity to Polycomb repression.

Genes & development·2025

Video Experimental Relacionado

Updated: Mar 2, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.5K

El bloqueo de la activación promiscua en los promotores crípticos dirige la expresión génica específica del tipo de

Jongmin Kim1,2, Chenggang Lu2, Shrividhya Srinivasan2

  • 1Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.

Science (New York, N.Y.)
|May 20, 2017
PubMed
Resumen

Kumgang (Kmg), una proteína de dedo de zinc, y dMi-2 impiden la transcripción genética somática en las células germinales masculinas de Drosophila. Esto asegura la activación genética adecuada durante el desarrollo mediante el bloqueo de la actividad promotora no deseada.

Más Videos Relacionados

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.1K
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

8.6K

Videos de Experimentos Relacionados

Last Updated: Mar 2, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.5K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.1K
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

8.6K

Área de la Ciencia:

  • Biología del desarrollo
  • La genética
  • Biología molecular

Sus antecedentes:

  • El mantenimiento del silenciamiento genético para los linajes celulares no expresados es crucial durante el desarrollo.
  • Los factores de transcripción específicos y los remodeladores de cromatina juegan un papel clave en la regulación de los patrones de expresión génica.

Objetivo del estudio:

  • Investigar el mecanismo por el cual Kumgang (Kmg) y dMi-2 regulan la expresión génica en el linaje de células madre germinales masculinas de Drosophila.
  • Comprender cómo Kmg impide la transcripción de los genes somáticos en las células germinales.

Principales métodos:

  • Utilizó el linaje de células madre germinales masculinas de Drosophila como sistema modelo.
  • Investigó la función de la proteína del dedo de zinc Kumgang (Kmg) y el remodelador de la cromatina dMi-2.
  • Se analizó la interacción entre Kmg, dMi-2 y el activador Aly.

Principales resultados:

  • Kmg, junto con dMi-2, suprime la transcripción de genes de linaje somático en las células germinales masculinas de Drosophila.
  • Kmg bloquea la transcripción de los promotores crípticos, evitando la activación genética inadecuada.
  • Este mecanismo asegura que el activador Aly se dirige específicamente a las transcripciones para la diferenciación de las células germinales masculinas.

Conclusiones:

  • Kmg actúa como un regulador crítico para mantener el silenciamiento genético en linajes celulares específicos.
  • El bloqueo de activadores promiscuos en promotores crípticos es esencial para la activación genética precisa durante la diferenciación terminal.
  • El complejo Kmg-dMi-2 protege la expresión génica específica de la línea germinal al prevenir la transcripción génica somática.