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El control de las funciones catalíticas de las enzimas de ADN dentro de las redes dinámicas constitucionales de las

Shan Wang¹, Liang Yue¹, Zohar Shpilt¹

¹Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.

Journal of the American Chemical Society

|June 20, 2017

Resumen

Los investigadores crearon sistemas catalíticos adaptables utilizando nanoestructuras de ácido nucleico. Estas redes dinámicas constitucionales (CDN) responden a los disparadores externos, imitando los sistemas biológicos mediante la regulación hacia arriba o hacia abajo de los componentes para las funciones sintonizables.

Área de la Ciencia:

Química de los sistemas
Química supramolecular
Biología Química

Sus antecedentes:

La imitación de complejas redes químicas dinámicas celulares es un desafío clave en la química de sistemas.
Las redes dinámicas constitucionales (CDN) ofrecen un enfoque versátil para emular los biosistemas a través de la autoadaptación a los desencadenantes ambientales.
Las nanoestructuras de ácido nucleico proporcionan una plataforma robusta para la construcción de sistemas moleculares sensibles.

Objetivo del estudio:

Construir redes dinámicas constitucionales desencadenadas por efectores (CDN) utilizando nanoestructuras de ácido nucleico.
Demostrar propiedades catalíticas adaptables dentro de estas CDN en respuesta a estímulos externos.
Investigar los mecanismos de regulación ascendente y descendente de los componentes de la CDN.

Principales métodos:

Construcción de las CDN utilizando cuatro componentes intercambiables de ácido nucleico (AA', BA', AB', BB').
Aplicación de moléculas efectoras (cadenas de combustible, iones K +) para estabilizar o desestabilizar selectivamente los componentes de la CDN a través de la formación triplex o G-cuadruplex.
Monitoreo de la reconfiguración de la red y de la actividad catalítica mediante electroforesis cuantitativa en gel y ensayos funcionales.

Principales resultados:

Se ha demostrado la regulación ascendente y descendente de los componentes de la CDN basada en la presencia de efectores.
Las propiedades catalíticas adaptables que surgen de las redes reconfiguradas dinámicamente.
Las CDN reconfiguradas con éxito a sus estados originales utilizando disparadores de contador (cadenas anti-combustible, éteres de corona).

Conclusiones:

Las CDN basadas en ácido nucleico pueden imitar efectivamente las redes biológicas dinámicas con funciones catalíticas sintonizables.
El control ortogonal y conmutable sobre los componentes de la CDN se puede lograr a través de moléculas efectoras y contra-gatillo específicas.
Este trabajo proporciona una base para el diseño de sistemas moleculares adaptativos sofisticados para diversas aplicaciones.

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