Video Experimental Relacionado
Updated: Feb 26, 2026
10:22
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
Published on: December 4, 2015
9.4K
El perfil funcional de un genoma de Plasmodium revela una abundancia de genes esenciales
Ellen Bushell1, Ana Rita Gomes1, Theo Sanderson1
1Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, UK.
Cell
|July 15, 2017
Resumen
Los parásitos de la malaria (Plasmodium berghei) tienen muchos genes esenciales, con dos tercios necesarios para el crecimiento. Este estudio identifica genes clave para el desarrollo de fármacos y explica los desafíos en la creación de vacunas debido a la redundancia funcional.
Área de la Ciencia:
- La genómica
- Parasitología
- Descubrimiento de drogas
Sus antecedentes:
- Los parásitos de la malaria poseen numerosos genes con funciones desconocidas.
- La identificación de genes esenciales es crucial para el desarrollo de nuevos medicamentos contra la malaria.
- Comprender la función genética y la redundancia es clave para combatir la malaria.
Objetivo del estudio:
- Identificar sistemáticamente los genes y las vías esenciales en Plasmodium berghei para el desarrollo de fármacos contra la malaria.
- Crear una base de datos completa de fenotipos de los mutantes knockout de Plasmodium berghei.
- Investigar el grado de esencialidad genética y redundancia funcional en parásitos de la malaria.
Principales métodos:
- Generó 2.578 mutantes de Plasmodium berghei con código de barras.
- Se midieron las tasas de crecimiento competitivo de los mutantes en un modelo de ratón.
- Compilé una base de datos de fenotipos para analizar la esencialidad de los genes.
Principales resultados:
- Más de dos tercios de los genes de Plasmodium berghei son esenciales para un crecimiento óptimo en una sola etapa del ciclo de vida.
- Esta alta proporción de genes esenciales no tiene precedentes en los organismos.
- Se observó una redundancia funcional significativa en las familias de genes involucrados en las interacciones parásito- huésped.
Conclusiones:
- La extensa esencialidad genética destaca el genoma optimizado de Plasmodium berghei para el parasitismo.
- La redundancia funcional puede explicar la eficacia variable del fármaco y los desafíos del desarrollo de vacunas.
- Los resultados proporcionan una base para el descubrimiento y las estrategias de intervención de fármacos antimaláricos específicos.
Videos de Conceptos Relacionados
01:15Diversity of Protists I
1.5K
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
1.5K
01:27Overview of Protists
2.3K
Protists are diverse eukaryotic microorganisms that lack the specialized tissues of plants and animals and the chitinous cell walls of fungi. Their early divergence within Eukarya resulted in structural, functional, and ecological diversity. They are classified into supergroups such as Archaeplastida, Excavata, Amoebozoa, Rhizaria, Alveolata, and Stramenopiles, determined through genetic analysis and structural similarities.Structural and Functional AdaptationsProtists have various adaptations...
2.3K
02:24Ribosome Profiling
4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
01:28Plasmids
3.3K
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
3.3K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
17.2K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
17.2K
01:27Diversity of Protists II
1.5K
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
1.5K