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Ajuste del tamaño de la cavidad y la quiralidad de los cristales de ADN 3D de autoensamblaje

Chad R Simmons, Fei Zhang, Tara MacCulloch

¹Department of Chemistry, New York University , New York, New York 10003, United States.

Journal of the American Chemical Society

|July 22, 2017

Resumen

Los investigadores desarrollaron una nueva nanoestructura de ADN mediante la vinculación de cuatro capas de doble hélice, creando cristales resistentes a la nucleasa. Este avance permite una disposición precisa en 3D de las moléculas invitadas dentro de la red de ADN.

Área de la Ciencia:

* Nanotecnología estructural del ADN
* Cristalografía
* Autoensamblaje biomolecular

Sus antecedentes:

* La nanotecnología del ADN utiliza oligonucleótidos para el autoensamblaje programable de nanoestructuras.
* Un objetivo clave es la construcción de redes de ADN 3D para la determinación de la estructura macromolecular.
* La naturaleza programable del ADN permite el diseño racional de cristales autoensamblados para inmovilizar moléculas huéspedes.

Objetivo del estudio:

* Para extender un motivo de ADN conocido (4 × 5) a un sistema de cuatro capas de doble hélice.
* Para crear cristales de imagen especular (l-ADN) resistentes a la nucleasa.
* Para determinar la estructura 3D precisa de estos nuevos ensamblajes de ADN.

Principales métodos:

* Ensamblaje de un nuevo motivo de ADN (4 × 6) utilizando un oligonucleótido de tejido central con cuatro repeticiones de seis bases.
* Formación de las uniones de Holliday para organizar la matriz en capas.
*Cristalización de las formas d-ADN y l-ADN, incluidos los derivados de bromo y selenio, para el análisis por difracción de rayos X.

Principales resultados:

* El ensamblaje exitoso de una estructura de ADN de cuatro capas de doble hélice.
* Cristalización del ADN-l resistente a la nucleasa y su homólogo de ADN-d.
* Resolución de las estructuras cristalinas a 3,0 y 3,05 Å, revelando cavidades bien definidas adecuadas para la encapsulación de moléculas invitadas.

Conclusiones:

* La nanoestructura de ADN desarrollada proporciona una matriz 3D altamente organizada con cavidades discretas.
* El sistema permite el ensamblaje a priori de conjugados de ADN huésped con mano cristalina controlada.
* Este trabajo avanza en la nanotecnología del ADN para un control estructural preciso y aplicaciones potenciales en el alojamiento molecular.

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