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Una vesícula exocítica trans derivada de Golgi puede contener tanto la colinesterasa recién sintetizada como la
Cell
|January 16, 1987
Resumen
Este estudio rastrea el movimiento de la transferrina (Tf) en las células del hígado de la rata utilizando un nuevo método. Los resultados muestran que Tf se mueve de las vesículas endocíticas a las vesículas exocíticas originadas en el trans Golgi, lo que indica su papel en el transporte de la superficie celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Hepatología Hepatología.
Sus antecedentes:
- La transferrina (Tf) es crucial para el transporte de hierro en las células.
- Las vías endocíticas y exocíticas median el tráfico intracelular.
- Las vesículas recubiertas (CV) son los principales intermediarios de transporte.
Objetivo del estudio:
- Para investigar el tráfico intracelular de transferrina internalizada (Tf).
- Para determinar el origen y la trayectoria de las vesículas revestidas exocíticas (CV) en el hígado de rata.
- Aclarar el papel de los CV en las transferencias en el transporte.
Principales métodos:
- Utilizó un protocolo de cambio de densidad mediado por colinesterasa.
- Empleados radiolabelados 125I-Tf para rastrear la internalización y el movimiento.
- Perfundido hígado de rata con asialotransferrina para investigar el origen de las vesículas.
Principales resultados:
- El 125I-Tf se encontró inicialmente en CVs endocíticos, no en CVs exocíticos.
- 125I-Tf apareció en CVs exocíticos entre 1 y 2 horas después de la internalización.
- Los CV exocíticos contenían Tf reanalizado, lo que confirmaba el origen trans Golgi.
- La cinética de Tf en CVs exocíticos coincidió con la liberación extracelular.
Conclusiones:
- Las vesículas recubiertas (CV) están involucradas en el transporte de transferrina.
- La red trans Golgi es el origen de los CV exocíticos portadores de Tf.
- Los CV exocíticos median la transferencia de Tf desde el trans Golgi a la superficie celular.
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