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Videos de Conceptos Relacionados

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...

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Video Experimental Relacionado

Updated: May 10, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

El compromiso de empalme dicta el procesamiento de ARN alternativo específico de la neurona en la expresión génica de

S E Leff, R M Evans, M G Rosenfeld

    Cell
    |February 13, 1987
    PubMed
    Resumen
    Este resumen es generado por máquina.

    El procesamiento de ARN específico de la célula del gen de la calcitonina/CGRP produce distintos ARN mensajeros (ARNm) en las neuronas y las células tiroideas. Este estudio sugiere que un factor neural dicta un empalme y una poliadenilación alternativos para la producción de ARNm CGRP.

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    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • La neurociencia es la neurociencia.
    • Genética La genética.

    Sus antecedentes:

    • El gen de la calcitonina/CGRP se somete a un procesamiento de ARN alternativo específico de la célula.
    • Esto da como resultado distintos ARN mensajeros (ARNm): el ARNm CGRP en las neuronas y el ARNm de calcitonina en las células C de la tiroides.
    • La poliadenilación alternativa y el empalme de exones son mecanismos clave que impulsan la diversidad de transcripciones.

    Objetivo del estudio:

    • Investigar los mecanismos reguladores subyacentes al procesamiento de ARN alternativo específico de la célula del gen de la calcitonina/CGRP.
    • Para identificar los factores o mecanismos responsables de la producción diferencial de ARNm en las neuronas frente a las células tiroideas.
    • Explorar el papel de los factores reguladores del compromiso de empalme en la generación de ARNm CGRP.

    Principales métodos:

    • Expresión de genes de tipo salvaje y mutados de calcitonina/CGRP en sistemas celulares heterólogos.
    • Análisis de las poblaciones de ARNm resultantes para determinar patrones de poliadenilación y empalme alternativos.
    • Estudios comparativos entre los patrones de expresión de las células neuronales y tiroideas.

    Principales resultados:

    • La expresión heteróloga producía predominantemente calcitonina o ARNm CGRP, dependiendo del tipo de célula.
    • La evidencia sugiere que las neuronas poseen maquinaria específica o factores que influyen en las vías alternativas de empalme.
    • Se observó una correlación entre las vías de empalme específicas y la selección de sitios alternativos de poli (A).

    Conclusiones:

    • Es probable que las neuronas expresen un factor regulador que dirige el empalme alternativo y la poliadenilación para la producción de ARNm CGRP.
    • Se plantea la hipótesis de que un factor regulador del compromiso de empalme modula la estructura del pre-ARNm.
    • Esta modulación puede revelar sitios de empalme crípticos esenciales para generar mRNA CGRP específico del cerebro.