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Activación fotoquímica de aminas terciarias para aplicaciones en el estudio de la fisiología celular

Naeem Asad¹, Davide Deodato¹, Xin Lan²

¹New York University Abu Dhabi , P.O. Box 129188, Abu Dhabi, United Arab Emirates.

Journal of the American Chemical Society

|August 15, 2017

Resumen

Los investigadores desarrollaron un nuevo compuesto fotoactivado utilizando el grupo protector fotomovible 8-ciano-7-hidroxiquinolinilo (CyHQ) (PPG). Esta innovación permite la liberación rápida y eficiente de moléculas bioactivas para estudios de fisiología celular.

Área de la Ciencia:

Química orgánica
La fotoquímica
Biología celular

Sus antecedentes:

Los grupos fotomovibles de protección (PPG) son herramientas cruciales para controlar la liberación de moléculas bioactivas en los sistemas biológicos.
El desarrollo de PPG eficientes y de acción rápida es esencial para el estudio de los procesos celulares dinámicos.
La fracción 8-ciano-7-hidroxiquinolinilo (CyHQ) ofrece potencial como un nuevo andamio de PPG.

Objetivo del estudio:

Síntesis y caracterización de nuevos compuestos fotoactivados mediante la vinculación de aminas terciarias al PPG CyHQ.
Evaluar la eficiencia y la cinética de la liberación de moléculas bioactivas tras la fotoactivación.
Para explorar la utilidad de estos compuestos en aplicaciones biológicas, como la activación de Cre recombinasa y CRISPR-Cas9.

Principales métodos:

Síntesis de aminas terciarias protegidas por CyHQ.
Estudios de fotoactivación con excitación de uno y dos fotones (1PE y 2PE).
Análisis espectroscópico con resolución de tiempo para determinar la cinética de la fotorrealización.

Principales resultados:

Se han creado con éxito formas fotoactivadas de aminas terciarias representativas utilizando el PPG CyHQ.
Se ha demostrado una liberación altamente eficiente de moléculas bioactivas, incluyendo tamoxifeno y 4-hidroxitamoxifeno, a través de 1PE y 2PE.
Se ha observado una liberación fotográfica extremadamente rápida de aminas terciarias (70 ps) desde el estado excitado singlet de CyHQ.

Conclusiones:

El CyHQ PPG es eficaz para crear aminas terciarias fotoactivadas con cinética de liberación rápida.
Estos compuestos son prometedores para aplicaciones en fisiología celular, edición de genes (CRISPR-Cas9) y activación de la Cre recombinasa.
El CyHQ PPG ofrece una herramienta valiosa para el control espacio-temporal de la entrega de moléculas bioactivas en la investigación biológica.

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