Video Experimental Relacionado

Updated: Feb 23, 2026

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

Liberación desencadenada de las vesículas bilaye de lípidos por un sistema de transducción de señales transmembrana

Matthew J Langton¹, Lorel M Scriven¹, Nicholas H Williams²

¹Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.

Journal of the American Chemical Society

|September 7, 2017

Resumen

Los investigadores desarrollaron liposomas intercambiables para la administración controlada de medicamentos. Un catalizador incrustado libera medicamentos encapsulados bajo demanda utilizando cambios de pH, abordando un desafío clave en la nanomedicina.

Área de la Ciencia:

Ciencia de los biomateriales
Nanotecnología
Sistemas de administración de fármacos

Sus antecedentes:

La liberación controlada de fármacos de los nanoportadores es crucial para las terapias efectivas.
El control espacio-temporal de la administración de fármacos sigue siendo un desafío importante en la medicina.

Objetivo del estudio:

Para diseñar vesículas de doble capa lipídica (liposomas) capaces de desencadenar la liberación de la carga.
Implementar un mecanismo de transducción de señal transmembrana artificial para el control externo.

Principales métodos:

Incorporación de un transductor de señal catalítico sintético sensible al pH en las membranas de los liposomas.
Catalización de la formación de surfactantes dentro de los liposomas en respuesta a los cambios de pH externos.
Monitoreo de la permeabilización inducida por el surfactante y de la liberación hidrofílica de la carga.

Principales resultados:

El transductor de señal sintético catalizó efectivamente la producción de surfactante al activar el pH externo.
La generación de surfactante condujo a la permeabilización de la membrana liposómica.
Se logró una liberación controlada de la carga hidrófila encapsulada en respuesta a estímulos de pH.

Conclusiones:

Los liposomas equipados con transductores de señales artificiales ofrecen una plataforma viable para la administración de medicamentos bajo demanda.
El control externo del pH permite la regulación espacio-temporal de la liberación de la carga molecular.
Este enfoque proporciona una nueva estrategia para aplicaciones de nanomedicina dirigidas y controladas.

Más Videos Relacionados

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Videos de Conceptos Relacionados

Insertion of Single-pass Transmembrane Proteins in the RER

Insertion of Single-pass Transmembrane Proteins in the RER

Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...

Fusion of Secretory Vesicles with the Plasma Membrane

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...

Amplifying Signals via Second Messengers

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...

Cell-surface Signaling

Cell-surface Signaling

Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.

Intracellular Signaling Cascades

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...