Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Homologous Recombination02:31

Homologous Recombination

64.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.1K
Homologous Recombination02:31

Homologous Recombination

6.9K
6.9K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

93.9K
93.9K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.4K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.4K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

15.3K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
15.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

4.5K
4.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Recent equatorward shift of the summer North Atlantic jet dominated by internal climate variability.

Science advances·2026
Same author

The Association Between Glycemic Disorders and Acute Coronary Syndrome: Plaque Vulnerability in Perimenopausal and Post-Menopausal Women With Diabetes.

British journal of hospital medicine (London, England : 2005)·2026
Same author

Diabetes May Modulate the Association Between Age and Optical Coherence Tomography Angiography Parameters: A Serial, Cross-Sectional Study.

Journal of personalized medicine·2026
Same author

Transmembrane Domain Dominance Drives Emergent Signaling and Allosteric Inversion in mGlu<sub>1/5</sub> Heterodimers.

bioRxiv : the preprint server for biology·2026
Same author

FBH1 and RAD54L directly interact and cooperate to drive replication fork reversal.

bioRxiv : the preprint server for biology·2026
Same author

Multifaceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection.

Nature communications·2026

Video Experimental Relacionado

Updated: Feb 21, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.2K

BRCA1-BARD1 promueve el emparejamiento de ADN homólogo mediado por RAD51

Weixing Zhao1, Justin B Steinfeld2, Fengshan Liang1,3,4

  • 1Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Nature
|October 5, 2017
PubMed
Resumen

El complejo supresor del tumor BRCA1-BARD1 es crucial para la reparación del ADN por recombinación homóloga, mejorando la actividad de la recombinasa RAD51. Este hallazgo revela un nuevo objetivo terapéutico para el tratamiento del cáncer.

Más Videos Relacionados

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.6K
Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 17, 2011

15.1K

Videos de Experimentos Relacionados

Last Updated: Feb 21, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.2K
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.6K
Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 17, 2011

15.1K

Área de la Ciencia:

  • Biología molecular
  • La genética
  • Investigación del cáncer

Sus antecedentes:

  • El complejo BRCA1-BARD1 es un supresor tumoral clave involucrado en la reparación de la ruptura de doble cadena de ADN a través de la recombinación homóloga.
  • Facilita la resección del extremo del ADN, creando una plantilla para otros complejos como BRCA2-PALB2 y RAD51.

Objetivo del estudio:

  • Para investigar la interacción entre BRCA1-BARD1 y RAD51.
  • Para aclarar el papel de BRCA1-BARD1 en la recombinación homóloga mediada por RAD51 y la reparación del ADN.

Principales métodos:

  • Purificación y examen de los complejos BRCA1-BARD1 de tipo silvestre y mutantes.
  • Ensayos bioquímicos para evaluar la unión al ADN, la interacción con RAD51 y la actividad de la recombinasa.
  • Ensayos celulares para evaluar la recombinación homóloga y la eficiencia de la reparación del ADN.

Principales resultados:

  • Tanto BRCA1 como BARD1 se unen al ADN e interactúan con el RAD51.
  • BRCA1-BARD1 mejora la actividad de la recombinasa RAD51 promoviendo el ensamblaje del complejo sináptico.
  • Los mutantes con interacciones BRCA1-BARD1/RAD51 debilitadas muestran una alteración en la formación de articulaciones de ADN y en la recombinación homóloga.

Conclusiones:

  • BRCA1-BARD1 juega un papel indispensable en la última etapa para estimular la actividad de RAD51 durante la recombinación homóloga.
  • Esta función es crítica para la reparación eficiente del ADN y la supresión del tumor.
  • Dirigirse a la interacción BRCA1-BARD1 con RAD51 presenta una estrategia potencial para el tratamiento del cáncer.