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La inhibición de la proliferación celular por los oligodeoxinucleótidos antisense al PCNA ciclina
D Jaskulski1, J K deRiel, W E Mercer
1Department of Pathology, Temple University Medical School, Philadelphia, PA 19140.
Science (New York, N.Y.)
|June 10, 1988
Resumen
El antígeno nuclear celular proliferante (PCNA) es crucial para la síntesis del ADN y la división celular. La inhibición del PCNA con oligodeoxinucleótidos antisense detiene la síntesis de ADN y la mitosis en las células.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- El antígeno nuclear de la célula proliferante (PCNA) es una proteína nuclear.
- El PCNA actúa como un cofactor para la ADN polimerasa delta.
Objetivo del estudio:
- Para investigar el papel de PCNA en la síntesis de ADN celular.
- Para determinar la importancia del PCNA en la progresión del ciclo celular.
Principales métodos:
- Se utilizaron oligodeoxinucleótidos antisentido para inhibir el PCNA en células Balb/c3T3 de crecimiento exponencial.
- Se administró un oligodeoxinucleótido de sentido como control.
Principales resultados:
- Los oligodeoxinucleótidos PCNA antisense suprimieron por completo la síntesis del ADN.
- La mitosis también fue completamente inhibida por los oligodeoxinucleótidos antisense PCNA.
- Los oligodeoxinucleótidos de Sense no mostraron efectos inhibidores.
Conclusiones:
- PCNA juega un papel vital en la síntesis de ADN celular.
- El PCNA es esencial para la progresión del ciclo celular.
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