Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Nucleosome Remodeling02:54

Nucleosome Remodeling

11.3K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.3K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.4K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.4K
Chromatin Packaging02:21

Chromatin Packaging

22.5K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.5K
Chromatin Packaging01:32

Chromatin Packaging

19.8K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
19.8K
Chromatin Packaging02:21

Chromatin Packaging

9.9K
9.9K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.5K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Transcription initiation drives dosage compensation establishment and maintenance in male <i>Drosophila</i>.

Science advances·2026
Same author

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same author

A modular framework for automated segmentation and analysis of AFM imaging of chromatin organization.

Nucleic acids research·2026
Same author

ELOF1 is a core component of the promoter-proximal paused RNA polymerase II complex.

bioRxiv : the preprint server for biology·2026
Same author

Structure and function of IWS1 in transcription elongation.

Nucleic acids research·2026
Same author

Mechanism of co-transcriptional cap snatching by influenza polymerase.

Nature·2026

Video Experimental Relacionado

Updated: Feb 21, 2026

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

2.3K

La estructura del nucleosoma-Chd1 y las implicaciones para la remodelación de la cromatina

Lucas Farnung1, Seychelle M Vos1, Christoph Wigge1

  • 1Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology, Am Fassberg 11, 37077 Göttingen, Germany.

Nature
|October 12, 2017
PubMed
Resumen

La proteína de unión de cromosoma-helicasa-ADN 1 (Chd1) remodela los nucleosomas al desprender el ADN y posicionar su motor ATPasa para la translocación. Esta visión estructural revela cómo Chd1 facilita la transcripción del ADN y mantiene la pluripotencia celular.

Más Videos Relacionados

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

23.2K
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
08:16

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Published on: December 29, 2021

3.3K

Videos de Experimentos Relacionados

Last Updated: Feb 21, 2026

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

2.3K
Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

23.2K
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
08:16

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Published on: December 29, 2021

3.3K

Área de la Ciencia:

  • Biología molecular
  • Biología estructural
  • La epigenética

Sus antecedentes:

  • Los factores de remodelación de la cromatina son cruciales para los procesos de ADN como la transcripción, la replicación y la reparación.
  • La proteína de unión de cromosoma-helicasa-ADN 1 (Chd1) conservada juega un papel en el espaciamiento de los nucleosomas, el paso de la ARN polimerasa II y la pluripotencia celular.

Objetivo del estudio:

  • Determinar la estructura por microscopía criolectrónica de la levadura Saccharomyces cerevisiae Chd1 unida a un nucleosoma.
  • Aclarar el mecanismo por el cual Chd1 remodela los nucleosomas e influye en la accesibilidad del ADN.

Principales métodos:

  • Microscopía cryoelectrónica con una resolución de 4,8 Å.
  • Análisis estructural del complejo Chd1-nucleosoma.

Principales resultados:

  • Chd1 separa dos giros de ADN del octámero de histona, uniéndose entre giros de ADN en un estado de equilibrio catalítico.
  • Los dominios específicos (SANT, SLIDE, motor ATPasa) interactúan con el ADN en ubicaciones superhélicas definidas (SHL) y la histona H4.
  • El cromodominio doble facilita el cierre de la ATPasa, lo que permite la translocación del ADN y la remodelación del nucleosoma.

Conclusiones:

  • La estructura revela el mecanismo de Chd1 para aflojar los giros del ADN y remodelar los nucleosomas.
  • La actividad de la ATPasa de Chd1, que potencialmente involucra un mecanismo de ratchet, es clave para su función en la regulación de la cromatina.
  • Esto proporciona una base estructural para el papel de Chd1 en la transcripción y la pluripotencia.