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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
Published on: June 28, 2018
Una proteína Ultrabithorax se une a secuencias cercanas a la suya y a los promotores de Antennapedia P1
P A Beachy1, M A Krasnow, E R Gavis
1Department of Biochemistry, Stanford University School of Medicine, California 94305.
Cell
|December 23, 1988
Resumen
La proteína Ultrabithorax (Ubx), crucial para el desarrollo de la mosca de la fruta, se une directamente a las secuencias de ADN cercanas a las suyas y a los promotores del gen Antenna-pedia (Antp). Esta unión sugiere que Ubx regula la actividad génica mediante la interacción con regiones específicas del ADN.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El gen Ultrabithorax (Ubx) en Drosophila codifica las proteínas que controlan la identidad del segmento.
- Las proteínas Ubx desempeñan un papel crítico en la especificación de los destinos de desarrollo de los segmentos torácico posterior y abdominal anterior.
- Los estudios genéticos indican que Ubx reprime el gen homeótico Antenna-pedia (Antp).
Objetivo del estudio:
- Investigar el mecanismo molecular por el cual las proteínas Ubx regulan la expresión génica.
- Para determinar si las proteínas Ubx son proteínas de unión al ADN e identificar sus dianas de ADN.
- Para aclarar la interacción directa entre las proteínas Ubx y las regiones reguladoras de los genes diana.
Principales métodos:
- Purificación de un miembro de la familia de proteínas Ubx de una cepa de Escherichia coli que produce en exceso.
- Caracterización de la proteína Ubx purificada como una proteína de unión al ADN específica de la secuencia.
- Identificación y análisis de secuencias de ADN unidas por la proteína Ubx, incluidas las cercanas a los promotores de Ubx y Antp.
Principales resultados:
- Un miembro de la proteína Ubx fue purificado con éxito de E. coli.
- La proteína Ubx purificada demostró actividad de unión al ADN específica de la secuencia.
- Se identificaron sitios de unión cerca de los promotores del gen Ubx y el promotor Antp P1.
- Estos sitios de unión, que van desde 40 a 90 bp, contienen repeticiones en tándem de TAA o TAA-TCG.
Conclusiones:
- Las proteínas Ubx regulan directamente la expresión génica a través de la unión de ADN específica de la secuencia.
- Los sitios de unión identificados sugieren un mecanismo directo para la regulación transcripcional mediada por Ubx.
- Estos hallazgos proporcionan una visión molecular del papel de Ubx en el patrón de desarrollo.
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