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Reprogramación epigenética embrionaria por un factor de transcripción pionero en las plantas
Zeng Tao1,2, Lisha Shen2, Xiaofeng Gu2
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai 201602, China.
Nature
|October 27, 2017
Resumen
La memoria epigenética de las plantas se restablece cada generación. LEAFY COTYLEDON1 (LEC1) reactiva la expresión génica del locus de floración C (FLC) en los embriones, estableciendo una memoria para el desarrollo post-embrionario.
Área de la Ciencia:
- Biología molecular de las plantas
- La epigenética
- Biología del desarrollo
Sus antecedentes:
- Las modificaciones epigenéticas regulan la expresión génica y el destino celular.
- Las marcas epigenéticas deben restablecerse en los gametos / embriones para el desarrollo adecuado de la descendencia.
- En Arabidopsis, el frío de invierno (vernalización) silencia el LOCUS FLOWERING C (FLC), un represor floral, pero este silencio se restablece cada generación.
Objetivo del estudio:
- Investigar el mecanismo de restablecimiento del silenciamiento epigenético de FLC en embriones de Arabidopsis.
- Comprender el papel de LEAFY COTYLEDON1 (LEC1) en el establecimiento de estados activos de cromatina en los embriones.
Principales métodos:
- Análisis de las modificaciones epigenéticas y la expresión génica en Arabidopsis thaliana.
- Centrarse en el papel del factor de transcripción LEAFY COTYLEDON1 (LEC1) durante la embriogénesis.
Principales resultados:
- LEAFY COTYLEDON1 (LEC1) promueve el establecimiento de un estado activo de cromatina en el FLC en los proembriones.
- LEC1 activa la expresión FLC de novo en los embriones, revirtiendo el silenciamiento heredado por los gametos.
- Este estado de cromatina activa embrionaria se transmite a las etapas post-embrionarias, creando una memoria epigenética.
Conclusiones:
- LEC1 reprograma los estados de la cromatina embrionaria en las plantas.
- Este mecanismo proporciona información sobre la memoria epigenética de la expresión génica activa desde las fases embrionarias hasta las posembrionarias.
- Un factor embrionario (LEC1) influye en el desarrollo posembrionario distinto de la embriogénesis.
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