Video Experimental Relacionado
Updated: Feb 20, 2026
06:02
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Published on: September 13, 2018
7.5K
Un lncRNA independiente del interferón promueve la replicación viral mediante la modulación del metabolismo celular
Pin Wang1, Junfang Xu2, Yujia Wang2
1National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
Science (New York, N.Y.)
|October 28, 2017
Resumen
Los investigadores descubrieron un ARN largo no codificante (ARNlnc) que los virus inducen para aumentar su replicación al mejorar una enzima metabólica. Este hallazgo ofrece un nuevo objetivo para las terapias antivirales de acción amplia.
Área de la Ciencia:
- Virología
- Biología molecular
- Regulación del metabolismo
Sus antecedentes:
- Los virus manipulan el metabolismo del huésped para replicarse.
- Las moléculas reguladoras clave que median estos cambios metabólicos durante la infección viral son en gran medida desconocidas.
- Comprender estas moléculas es crucial para desarrollar estrategias antivirales.
Objetivo del estudio:
- Identificar nuevas moléculas huésped que regulan las redes metabólicas durante la infección viral.
- Aclarar el mecanismo por el cual un ARN no codificante largo específico (ARNlnc) influye en la replicación viral.
- Para explorar el potencial de dirigirse a este lncRNA para la terapia antiviral.
Principales métodos:
- Inducción de lncRNA por múltiples virus en células de ratón y humanas.
- Evaluación de la replicación viral en células con y sin lncRNA-ACOD1.
- Investigación de la unión del lncRNA-ACOD1 a las enzimas metabólicas mediante ensayos bioquímicos.
- Evaluación del impacto de la proteína GOT2 y sus metabolitos en la replicación viral in vivo.
Principales resultados:
- Se identificó un nuevo lncRNA, lncRNA-ACOD1, que es inducido por varios virus independientemente del interferón de tipo I (IFN-I).
- La deficiencia de lncRNA-ACOD1 redujo significativamente la infección viral in vivo a través de vías independientes de IFN-I-IRF3.
- lncRNA-ACOD1 se une directamente y mejora la actividad catalítica de la enzima metabólica GOT2.
- La proteína GOT2 y sus metabolitos podrían rescatar la replicación viral en ausencia de lncRNA-ACOD1, aumentando la letalidad.
Conclusiones:
- El lncRNA-ACOD1 inducido por el virus promueve la replicación viral al mejorar la actividad de las enzimas metabólicas del huésped.
- Esto representa un nuevo mecanismo de retroalimentación que vincula el lncRNA, el metabolismo y la propagación viral.
- lncRNA-ACOD1 y su interacción con GOT2 presentan un objetivo terapéutico potencial para tratamientos antivirales de amplio espectro.
Videos de Conceptos Relacionados
02:39lncRNA - Long Non-coding RNAs
10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
02:39lncRNA - Long Non-coding RNAs
3.7K
3.7K
01:23RNA Interference
28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
01:29Viruses with RNA Genomes
1.0K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.0K
02:30siRNA - Small Interfering RNAs
18.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.8K
01:19Regulation of Metabolism
11.8K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.8K