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Secuenciación de la cadena de sulfato de Dermatan de Decorin

Yanlei Yu¹, Jiana Duan², Franklin E Leach²

¹School of Food Science and Biotechnology, Zhejiang Gongshang University , Hangzhou, Zhejiang 310018, China.

Journal of the American Chemical Society

|November 8, 2017

Resumen

Los investigadores determinaron la estructura compleja del proteoglicano de la decorina

Área de la Ciencia:

Productos glicémicos
Estructura de los proteoglicanos
Análisis de los glucosaminoglicanos

Sus antecedentes:

El control de la glucosilación es poco conocido, especialmente para los proteoglicanos complejos (PG).
Las cadenas laterales de los glucosaminoglicanos (GAG) son estructuralmente complejas.
Trabajos previos aclararon la cadena de sulfato de condroitina de la bikunin PG.

Objetivo del estudio:

Determinar la estructura compleja de la cadena GAG del sulfato de dermatan de la decorina PG.
Para identificar los motivos estructurales dentro de la cadena GAG de Decorin.
Para comparar la complejidad estructural de diferentes GAG.

Principales métodos:

Técnicas sofisticadas de separación.
Análisis de composición y mapeo de dominio.
Espectrometría de masas en tándem con un algoritmo genético modificado.

Principales resultados:

Se aclaró el motivo estructural de la cadena GAG de decorin dermatan sulfate.
Este es el segundo GAG identificado con un motivo estructural prominente.
La estructura GAG de Decorin es menos variable de lo que se había anticipado.

Conclusiones:

La determinación estructural del GAG del sulfato de dermatan de la decorina proporciona información clave sobre la complejidad del GAG.
La identificación de los motivos estructurales simplifica la comprensión de la variabilidad de los GAG.
Este estudio avanza en el campo de la investigación de los glicómicos y los proteoglicanos.

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