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Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin
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Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

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Dinámica del transporte de ATPasa tipo P revelada por el FRET de una sola molécula

Mateusz Dyla1,2,3, Daniel S Terry4, Magnus Kjaergaard1,2,3,5

  • 1Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation & Danish Research Institute of Translational Neuroscience - DANDRITE, Nordic-EMBL Partnership for Molecular Medicine, Aarhus University, DK-8000 Aarhus C, Denmark.

Nature
|November 17, 2017
PubMed
Resumen

Los investigadores observaron directamente cambios conformacionales en las ATPasas de tipo P transportadoras de calcio utilizando FRET de una sola molécula. El eflujo de calcio está limitado por la formación de fosfoenzimas, revelando intermediarios de transporte clave.

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