Electroforesis 2D analizada por computadora con análisis en 2D
Video Experimental Relacionado
Updated: Nov 6, 2026
14:43
Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Published on: November 25, 2008
M R Krauss1, P J Collins, S H Blose
1Protein Databases, Inc., Huntington Station, New York 11746.
Nature
|February 16, 1989
Resumen
Un nuevo servicio analiza los geles 2D y los compara con bases de datos, lo que hace que los estudios de expresión de proteínas sean más accesibles a los laboratorios de investigación de todo el mundo.
Área de la Ciencia:
- La proteómica es la proteómica.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La electroforesis de gel bidimensional (2D-PAGE) es una técnica clave para el análisis de la expresión de proteínas.
- El acceso a herramientas avanzadas de análisis ha sido una limitación para muchos laboratorios.
Objetivo del estudio:
- Introducir un nuevo servicio para el análisis de datos de electroforesis en gel 2D.
- Para facilitar la comparación de los resultados experimentales con las bases de datos de expresión de proteínas existentes.
Principales métodos:
- Utilizando algoritmos computacionales avanzados para el análisis de imágenes de gel 2D.
- Implementación de un módulo de comparación de bases de datos para la identificación y cuantificación de proteínas.
Principales resultados:
- El servicio permite un análisis exhaustivo de los geles 2D.
- Facilita la proteómica comparativa en diferentes estudios y laboratorios.
Conclusiones:
- Este servicio democratiza el análisis avanzado de gel 2D.
- Amplía significativamente el alcance de las capacidades de investigación de expresión de proteínas para los laboratorios a nivel mundial.
Videos de Conceptos Relacionados
02:35DNA Agarose Gel Electrophoresis
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
01:27SDS-PAGE
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
01:22Two-dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
01:20Electrophoresis: Overview
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
01:20Capillary Electrophoresis: Instrumentation
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
01:30Capillary Electrophoresis: Applications
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...