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Estructura del canal iónico sensible al frío y al mentol TRPM8
Ying Yin1, Mengyu Wu2, Lejla Zubcevic1
1Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710, USA.
Science (New York, N.Y.)
|December 9, 2017
Resumen
Los investigadores han descubierto la estructura del mosquitero de cuello en el microscopio cryoelectrónico
Área de la Ciencia:
- Biología estructural
- Investigación de los canales iónicos
- Neurociencia sensorial
Sus antecedentes:
- Los canales de melastatina potencial receptor transitorio (TRPM) son sensores polimodales cruciales en los procesos fisiológicos.
- TRPM8 se identifica como el sensor primario para el resfriado y el mentol en humanos.
Objetivo del estudio:
- Determinar la estructura de la criomicroscopia del canal TRPM8 de toda su longitud.
- Para aclarar la base estructural de la sensación de frío y mentol.
Principales métodos:
- Para determinar la estructura se empleó la crio-microscopía electrónica (crio-EM).
- Análisis estructural de alta resolución del canal TRPM8.
Principales resultados:
- La estructura TRPM8 exhibe una arquitectura distinta de tres capas.
- Se observó un nuevo pliegue del dominio amino-terminal y extensas interacciones en el anillo citosólico.
- Se propone que el sitio de unión de mentol resida dentro del dominio similar al sensor de tensión.
Conclusiones:
- La estructura TRPM8 determinada proporciona información sobre la arquitectura molecular de un canal sensorial clave.
- Esta información estructural ofrece una visión del mecanismo de transducción de la sensación de frío y mentol.
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