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Nanotubo de proteína selectivamente cleable con ADN: polimerización supramolecular de las "chaperonas moleculares
Daiki Kashiwagi1, Seunghyun Sim1, Tatsuya Niwa2
1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Journal of the American Chemical Society
|December 12, 2017
Resumen
Los investigadores diseñaron nanotubos de proteínas utilizando GroEL funcionalizado con ADN (chaperones moleculares diseñados genéticamente). Estas nuevas nanoestructuras de proteína-ADN exhiben una notable estabilidad y potencial para aplicaciones de entrega dirigidas.
Área de la Ciencia:
- La bioquímica
- Nanotecnología
- Biología molecular
Sus antecedentes:
- Las chaperonas moleculares como GroEL pueden ser diseñadas para nuevas funciones.
- La nanotecnología del ADN ofrece un control preciso sobre el ensamblaje a nanoescala.
Objetivo del estudio:
- Para diseñar y sintetizar nanotubos de proteínas utilizando GroEL funcionalizado con ADN.
- Investigar la estabilidad termodinámica y las propiedades de desmontaje de estas nuevas nanoestructuras.
- Para explorar el potencial de estos nanotubos de proteínas para la entrega dirigida.
Principales métodos:
- Ingeniería de proteínas GroEL para llevar múltiples hebras de ADN en sus dominios apicales.
- El ensamblaje de GroEL funcionalizados para formar nanotubos de proteínas unidimensional (NT).
- Investigación de la estabilidad y desmontaje de NT utilizando hebras de ADN complementarias y parcialmente complementarias.
Principales resultados:
- Se han preparado con éxito los conjugados de GroEL-ADN y se han ensamblado en nanotubos de proteínas estables (NT10a/10b y NT15c/10d).
- Se ha demostrado una estabilidad termodinámica anómala en NT10a/10b debido a la alta multivalencia.
- Se ha observado un desmontaje selectivo y rápido de NT15c/10d tras la introducción de ADN específico (15d), lo que pone de manifiesto la posibilidad de liberación controlada.
Conclusiones:
- Los conjugados de GroEL-ADN diseñados permiten la formación de nanotubos de proteínas altamente estables.
- El desmontaje de estos nanotubos se puede controlar con precisión a través de la hibridación del ADN, lo que indica el potencial de sistemas de entrega dirigidos.
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