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Los péptidos RALF4/19 interactúan con las proteínas LRX para controlar el crecimiento del tubo de polen en

Martin A Mecchia^1,2,3, Gorka Santos-Fernandez¹, Nadine N Duss¹

¹Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland.

Science (New York, N.Y.)

|December 16, 2017

Resumen

El crecimiento del tubo de polen de Arabidopsis se basa en los péptidos del FACTOR DE ALKALINIZACIÓN RÁPIDA (RALF) y las proteínas REPEAT EXTENSIN (LRX) RICH en leucina. Estos interactúan para detectar cambios en la pared celular, señalando internamente a través de la vía CrRLK1L para un crecimiento sostenido.

Área de la Ciencia:

Biología vegetal
Señales celulares
Ciencias de las plantas moleculares

Sus antecedentes:

La comunicación celular con la matriz extracelular es vital para la función celular.
Las quinasas similares a los receptores (CrRLK1L) interactúan con los péptidos del Factor de Alcalinización Rápida (RALF), pero los mecanismos de percepción no están claros.
Las proteínas extensinas ricas en leucina (LRX) están involucradas en el desarrollo de la pared celular, pero su función no se comprende completamente.

Objetivo del estudio:

Investigar las funciones de Arabidopsis RALF4 y RALF19 en el desarrollo del tubo de polen.
Para aclarar el mecanismo por el cual los péptidos RALF son percibidos por la célula.
Para entender la función de las proteínas LRX en el crecimiento del tubo de polen.

Principales métodos:

El análisis genético de los mutantes de Arabidopsis.
Pruebas de crecimiento del tubo de polen.
Estudios de interacción entre proteínas.

Principales resultados:

Arabidopsis RALF4 y RALF19 regulan redundantemente la integridad y el crecimiento del tubo de polen.
La función de RALF4 y RALF19 depende de las proteínas LRX expresadas por el polen.
Las proteínas LRX interactúan con las RALF y controlan el estado de la pared celular.

Conclusiones:

Las proteínas LRX actúan como receptores para los péptidos RALF en el tubo de polen.
La interacción RALF-LRX señala cambios en la pared celular en la vía CrRLK1L.
Esta vía de señalización es esencial para mantener el crecimiento del tubo de polen.

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