Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Net Torque Calculations01:19

Net Torque Calculations

When a mechanic tries to remove a hex nut with a wrench, it is easier if the force is applied at the farthest end of the wrench handle. The lever arm is the distance from the pivot point (the hex nut in this case) to the person’s hand. If this distance is large, the torque is higher. Only the component of the force perpendicular to the lever arm contributes to the torque. Therefore, pushing the wrench perpendicular to the lever arm is more advantageous. If multiple people apply force to rotate...
Design Example: Capacitance Multiplier Circuit01:20

Design Example: Capacitance Multiplier Circuit

In integrated circuit technology, a capacitance multiplier is often utilized to produce a larger capacitance value when a small physical capacitance falls short. This is achieved by a circuit that multiplies capacitance values by a factor of up to 1000, such that a 10-pF capacitor can replicate the performance of a 100-nF capacitor.
The circuit illustrated in Figure 1 below incorporates two op-amps, with the first operating as a voltage follower and the second acting as an inverting amplifier.
PD Controller: Design01:26

PD Controller: Design

In automotive engineering, car suspension systems often employ Proportional Derivative (PD) controllers to enhance performance. PD controllers are utilized to adjust the damping force in response to road conditions. A controller, acting as an amplifier with a constant gain, demonstrates proportional control, with output directly mirroring input.
Designing a continuous-data controller requires selecting and linking components like adders and integrators, which are fundamental in Proportional,...
Dosage Regimen Designs: Nomograms and Tabulations01:23

Dosage Regimen Designs: Nomograms and Tabulations

Nomograms and tabulations are vital tools used by clinicians to design accurate and individualized dosage regimens. These instruments provide a straightforward method for adjusting dosages based on individual patient characteristics, including age, weight, and physiological condition. The foundation of a drug's nomogram is population pharmacokinetic data collected and analyzed using specific models. This data simplifies complex equations, presenting them diagrammatically or tabularly for easy...
Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Orally available designed miniproteins inhibit enterotoxigenic <i>Bacteroides fragilis</i> pathology by blocking toxin receptor binding.

bioRxiv : the preprint server for biology·2026
Same author

Membrane protein solubilization and structure determination using de novo-designed proteins.

Science (New York, N.Y.)·2026
Same author

Controlling metal-carbonate phase, form, and function through de novo protein design.

bioRxiv : the preprint server for biology·2026
Same author

Programmed synthesis of mesoporous protein crystals in cellular reactors.

Nature nanotechnology·2026
Same author

Generative design of programmable asymmetric β-barrel nanopores.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: De novo design of quasisymmetric two-component protein cages.

Nature·2026

Video Experimental Relacionado

Updated: May 11, 2026

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.5K

Diseño computacional completo de macrociclos de péptidos ordenados

Parisa Hosseinzadeh1, Gaurav Bhardwaj1, Vikram Khipple Mulligan1

  • 1Department of Biochemistry and Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.

Science (New York, N.Y.)
|December 16, 2017
PubMed
Resumen

Los investigadores desarrollaron un método computacional para diseñar nuevos macrociclos de péptidos de quiralidad mixta. Este enfoque amplía enormemente la diversidad estructural disponible para el descubrimiento de medicamentos y el desarrollo terapéutico.

Más Videos Relacionados

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

11.2K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.2K

Videos de Experimentos Relacionados

Last Updated: May 11, 2026

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.5K
Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

11.2K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.2K

Área de la Ciencia:

  • Química medicinal
  • Química computacional
  • Biología estructural

Sus antecedentes:

  • Los macrociclos de péptido de quiralidad mixta, como la ciclosporina, son potentes terapéuticos.
  • Los métodos existentes carecen de enfoques sistemáticos para explorar la diversidad estructural de estos compuestos.
  • Las proteínas naturales son malas plantillas para diseñar nuevos macrociclos de péptidos.

Objetivo del estudio:

  • Desarrollar una estrategia computacional para enumerar estructuras estables de macrociclos de péptidos de quiralidad mixta.
  • Para diseñar nuevos andamios de macrocíclos de péptidos para el descubrimiento de fármacos.
  • Para validar las predicciones computacionales con estructuras experimentales.

Principales métodos:

  • Muestreo casi exhaustivo de la columna vertebral de péptidos macrocíclicos.
  • Diseño de secuencias y cálculos de paisaje energético.
  • Determinación de la estructura por resonancia magnética nuclear (RMN).

Principales resultados:

  • Se identificaron más de 200 macrociclos diseñados que se prevé que adopten estructuras estables únicas.
  • Las estructuras experimentales de los macrociclos diseñados coincidían estrechamente con los modelos computacionales.
  • Se ha demostrado una expansión significativa de los andamios macrocíclicos de péptidos disponibles.

Conclusiones:

  • El método computacional desarrollado proporciona una cobertura completa del espacio estructural del macrociclo de péptidos cortos.
  • Este trabajo aumenta enormemente los andamios iniciales para el diseño racional de medicamentos y la selección de bibliotecas.
  • Permite la exploración y el diseño sistemáticos de nuevos macrociclos de péptidos terapéuticos.