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Updated: Feb 16, 2026
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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
Published on: May 25, 2017
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La trehalosa en la dieta aumenta la virulencia de la epidemia de Clostridium difficile
J Collins1, C Robinson2, H Danhof1
1Baylor College of Medicine, Department of Molecular Virology and Microbiology, One Baylor Plaza, Houston, Texas 77030, USA.
Nature
|January 9, 2018
Resumen
Dos cepas de Clostridium difficile (RT027 y RT078) evolucionaron para metabolizar el azúcar trehalosa. Esta adaptación, vinculada a la trehalosa como aditivo alimentario, puede explicar su mayor prevalencia y virulencia.
Área de la Ciencia:
- Microbiología
- Enfermedades infecciosas
- Biología molecular
Sus antecedentes:
- La infección por Clostridium difficile (CDI) es un patógeno importante asociado a la atención médica.
- No se comprende completamente la aparición de los ribotipos hipervirulentos de Clostridium difficile (RT027 y RT078).
Objetivo del estudio:
- Investigar los mecanismos detrás de la aparición y la hipervirulencia de las cepas epidémicas de Clostridium difficile.
- Determinar el papel del metabolismo de la trehalosa en la evolución de Clostridium difficile.
Principales métodos:
- Análisis genómico de los ribotipos epidémicos de Clostridium difficile.
- Ensayos bioquímicos para evaluar el metabolismo de la trehalosa.
- Modelo de ratón in vivo de la infección por Clostridium difficile.
Principales resultados:
- Las cepas RT027 presentan una mayor sensibilidad a la trehalosa debido a una mutación en el represor de la trehalosa.
- La trehalosa en la dieta aumenta la virulencia de RT027 en un modelo de ratón.
- Las cepas RT078 poseen un grupo de genes que permite el crecimiento en bajas concentraciones de trehalosa.
Conclusiones:
- La adquisición de las vías de metabolismo de la trehalosa es una adaptación clave para las cepas epidémicas de Clostridium difficile.
- El aumento de la trehalosa en la dieta probablemente fue seleccionado para estas cepas, contribuyendo a su aparición e hipervirulencia.
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