Video Experimental Relacionado

Updated: Feb 15, 2026

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

Published on: November 17, 2017

Escisión enzimática de péptidos ramificados para dirigirse a las mitocondrias

Hongjian He¹, Jiaqing Wang¹, Huaimin Wang¹

¹Department of Chemistry, Brandeis University , Waltham, Massachusetts 02454, United States.

Journal of the American Chemical Society

|January 13, 2018

Resumen

Este estudio introduce un nuevo método para atacar las mitocondrias utilizando péptidos cargados negativamente que se autoensamblan en nanofibras después de la escisión enzimática. Este enfoque permite una entrega eficiente de carga a las mitocondrias, superando las limitaciones de las moléculas catiónicas lipofílicas actuales.

Área de la Ciencia:

Ingeniería biomédica
Nanotecnología
Biología celular

Sus antecedentes:

Las moléculas dirigidas a las mitocondrias son a menudo lipofílicas y catiónicas, lo que plantea riesgos de citotoxicidad.
Existe la necesidad de estrategias más seguras y efectivas de orientación de las mitocondrias en la biomedicina.

Objetivo del estudio:

Desarrollar un nuevo sistema de autoensamblaje enzimático para el suministro de mitocondrias dirigidas.
Para demostrar la eficacia de este sistema para la entrega de carga intracelular a las mitocondrias.

Principales métodos:

Conjugación de la etiqueta FLAG con motivos de autoensamblaje para formar micelas.
Clivado enzimático del motivo FLAG por la enterokinasa (ENTK) para inducir la transformación en nanofibras.
Estudios de absorción celular y evaluaciones de la entrega de la carga.

Principales resultados:

Las micelas se transforman en nanofibras tras la acción de la ENTK intracelular, localizándose principalmente en las mitocondrias.
Las cargas entregadas por las micelas se dirigieron eficientemente a las mitocondrias en 2 horas.
La inhibición de la proteólisis ENTK redujo significativamente el objetivo de las mitocondrias.

Conclusiones:

El autoensamblaje enzimático ofrece una estrategia fundamentalmente nueva para la orientación de los orgánulos subcelulares.
Este método proporciona una alternativa más segura a los agentes lipofílicos catiónicos dirigidos a las mitocondrias.
El sistema demuestra potencial para la administración avanzada de fármacos y proteínas intracelulares a las mitocondrias.

Más Videos Relacionados

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Published on: March 7, 2025

Videos de Conceptos Relacionados

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

Peroxisomes and Mitochondria

Peroxisomes and Mitochondria

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...

Peptide Bonds

Peptide Bonds

A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Translocation of Proteins into the Mitochondria

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

Oxidative Cleavage of Alkenes: Ozonolysis

Oxidative Cleavage of Alkenes: Ozonolysis

In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.