Video Experimental Relacionado
Updated: Feb 14, 2026
06:59
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
Published on: August 11, 2010
12.5K
La redundancia del potenciador proporciona robustez fenotípica en el desarrollo de los mamíferos
Marco Osterwalder1, Iros Barozzi1, Virginie Tissières2,3
1Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
Nature
|February 9, 2018
Resumen
Los genomas de los mamíferos poseen numerosos potenciadores de genes que proporcionan funciones redundantes. Esta redundancia genética asegura la robustez fenotípica, previniendo anomalías del desarrollo cuando se pierden potenciadores individuales.
Área de la Ciencia:
- La genómica
- Biología del desarrollo
- Biología molecular
Sus antecedentes:
- Los genomas de los mamíferos contienen numerosos potenciadores específicos de los tejidos que regulan la expresión génica.
- No se comprende bien la importancia funcional de esta compleja red reguladora, en particular la redundancia de potenciadores.
Objetivo del estudio:
- Investigar la importancia funcional de múltiples potenciadores con actividades similares cerca del mismo gen.
- Determinar si la redundancia del potenciador confiere robustez fenotípica a las mutaciones de pérdida de función.
Principales métodos:
- Utilizó la edición del genoma para crear 23 líneas de eliminación de ratones dirigidas a potenciadores del desarrollo de las extremidades.
- Se han realizado eliminaciones de potenciadores individuales y combinatorios.
- Datos epigenómicos y transcriptómicos integrados de 29 tejidos de desarrollo de ratones.
Principales resultados:
- Las eliminaciones de potenciadores individuales de extremidades no causaron cambios morfológicos notables.
- La eliminación de pares de potenciadores de extremidades dio lugar a fenotipos de extremidades discernibles, lo que indica redundancia funcional.
- Los genes se asocian comúnmente con múltiples potenciadores que exhiben una actividad espacial temporal similar, proporcionando un amortiguador regulador.
Conclusiones:
- La redundancia del potenciador es una característica generalizada en los genomas de los mamíferos.
- Esta redundancia proporciona robustez fenotípica mediante el amortiguamiento contra la pérdida de potenciadores individuales.
- La redundancia del potenciador asegura la expresión genética normal y evita las consecuencias fenotípicas perjudiciales durante el desarrollo.
Más Videos Relacionados
Videos de Conceptos Relacionados
03:00Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
01:16Bioavailability Enhancement: Drug Solubility Enhancement
278
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
278
01:27Bioavailability Enhancement: Drug Permeability Enhancement
213
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
213
01:05Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
226
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
226
02:27Background and Environment Affect Phenotype
7.8K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.8K
01:43Sustainable Development
15.2K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
15.2K