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Oocitos hechos a medida para clonar primates no humanos

Jose B Cibelli1, John B Gurdon2

  • 1Departments of Animal Science and Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.

Cell
|February 10, 2018
PubMed
Resumen

Los investigadores lograron clonar con éxito a dos monos macacos mediante el uso de fibroblastos fetales. Este avance en la transferencia de núcleos de células somáticas (SCNT) se logró mejorando las modificaciones epigenéticas en los óvulos.

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Área de la Ciencia:

  • Biología de la reproducción
  • Biología del desarrollo
  • Investigaciones en primates

Sus antecedentes:

  • La transferencia nuclear de células somáticas (SCNT) es una técnica para la clonación de mamíferos.
  • La eficiencia de SCNT en primates sigue siendo baja debido a obstáculos en el desarrollo temprano.
  • La reprogramación epigenética es crucial para el éxito de la SCNT.

Objetivo del estudio:

  • Establecer un método SCNT más eficiente para los primates.
  • Para superar las barreras epigenéticas en el desarrollo temprano de SCNT.
  • Para producir primates clonados y sanos.

Principales métodos:

  • Se utilizaron fibroblastos fetales como células donantes.
  • Modificadores epigenéticos mejorados artificialmente en el ovocito.
  • Realizó transferencia de núcleos de células somáticas en monos macacos.

Principales resultados:

  • Se han clonado con éxito dos monos macacos sanos.
  • Demostró la viabilidad de la técnica SCNT mejorada.
  • Superó obstáculos críticos de desarrollo temprano en SCNT.

Conclusiones:

  • La modificación epigenética mejorada en los ovocitos mejora significativamente las tasas de éxito de SCNT en los primates.
  • Este estudio presenta un método viable para la clonación de primates.
  • Los hallazgos allanan el camino para nuevos avances en las tecnologías reproductivas de los primates.