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Kinetic Energy00:23

Kinetic Energy

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Kinetic energy is the ability of an object in motion to do work or enact change. It can take on many forms. For instance, water flowing down a waterfall has kinetic energy. In biological systems, particles of light travel and are absorbed by plants to create chemical energy. Animals consume the chemical energy and give off molecules that carry their scent through the air. They also generate kinetic energy when they run away from predators. Entire systems also possess kinetic energy, like the...
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Enzyme Kinetics01:19

Enzyme Kinetics

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
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Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy03:07

Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy

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The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws.
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Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

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Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
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Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)01:16

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

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Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...
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Actin Polymerization01:42

Actin Polymerization

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Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
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Video Experimental Relacionado

Updated: Feb 14, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

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Medición de la cinética de la polimerización interfacial mediante interferometría microfluídica

Arash Nowbahar1, Vincent Mansard2, Jodi M Mecca3

  • 1Department of Chemical Engineering , University of California , Santa Barbara , California 93106 , United States.

Journal of the American Chemical Society
|February 13, 2018
PubMed
Resumen

Se midió la cinética de polimerización interfacial para las películas de poliamida. Un nuevo método de interferometría microfluídica reveló una capa límite de reacción-difusión que controla la velocidad de reacción inicial.

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Área de la Ciencia:

  • Química de los polímeros
  • Ciencias de los materiales
  • Ingeniería Química

Sus antecedentes:

  • La polimerización interfacial es crucial para producir varios materiales como fibras, cápsulas y películas.
  • Las mediciones cinéticas precisas para la polimerización interfacial son difíciles debido a las películas delgadas y las reacciones rápidas.

Objetivo del estudio:

  • Investigar la cinética de la reacción de formación de la película de poliamida mediante técnicas avanzadas.
  • Para superar los desafíos de medición asociados con películas delgadas y velocidades de reacción rápidas en la polimerización interfacial.

Principales métodos:

  • Se utilizó la interferometría microfluídica para controlar los perfiles de concentración de monómeros en la interfaz de reacción.
  • Técnicas de medición in situ aplicadas para capturar cambios dinámicos durante la polimerización.

Principales resultados:

  • Se han medido con éxito los perfiles de concentración de monómeros cerca de la interfaz durante la formación de la película de poliamida.
  • Identificó una capa límite de reacción-difusión en la fase orgánica como el paso que limita la velocidad en la fase de reacción inicial.
  • Se obtuvo la primera constante de velocidad informada para este sistema de polimerización interfacial de poliamida específico.

Conclusiones:

  • La interferometría microfluídica es una técnica viable para estudiar la cinética de la polimerización interfacial rápida.
  • La capa límite de reacción-difusión influye significativamente en las etapas iniciales de la formación de la película de poliamida.
  • Este estudio proporciona datos cinéticos fundamentales para la polimerización interfacial, ayudando en la optimización del proceso y el diseño de materiales.