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Un enfoque versátil para los macrociclos de péptidos no canónicos y dinámicos covalentes de bucle único y múltiple
James F Reuther1, Andrew C Goodrich2, P Rogelio Escamilla1
1Department of Chemistry , University of Texas at Austin , Austin , Texas 78712 , United States.
Journal of the American Chemical Society
|February 22, 2018
Resumen
Los investigadores desarrollaron un nuevo método para crear macrociclos complejos de péptidos (MC) para agentes antimicrobianos mejorados. Esta técnica mejora la eficacia de los péptidos antimicrobianos existentes mediante la formación de bucles únicos, dobles y triples.
Área de la Ciencia:
- La bioquímica
- Química medicinal
- Ciencias de los materiales
Sus antecedentes:
- Los oligómeros peptídicos son andamios prometedores para los agentes antimicrobianos debido a su biocompatibilidad y afinidad.
- La formación de macrociclos complejos de péptidos (MC) con múltiples bucles es un desafío significativo en el desarrollo terapéutico.
Objetivo del estudio:
- Desarrollar un método versátil para crear macrociclos de péptidos de bucle simple, doble y triple (MC).
- Mejorar las propiedades antimicrobianas de los péptidos utilizando estas nuevas estructuras macrocíclicas.
Principales métodos:
- Se utiliza la condensación dinámica de hidrazona con dialdehído o dihidrazida.
- Péptidos sintetizados con cadenas laterales que contienen aldehído en resinas C-terminales de hidrazida para ensamblaje en resina.
- Se ha confirmado la formación de macrociclos mediante MALDI-TOF MS, HPLC y SDS-PAGE.
Principales resultados:
- Se han formado con éxito MC péptidos de bucle simple, doble y triple.
- Se obtienen conjuntos de péptidos MC sintonizables directamente después de la escisión de la resina.
- Se ha demostrado una mejora significativa de las propiedades antimicrobianas de la buforina II cuando se incorpora a estructuras macrocíclicas.
Conclusiones:
- La química covalente dinámica ofrece un enfoque versátil para construir macrociclos complejos de péptidos.
- La macrociclado de péptidos a través de la condensación de hidrazona aumenta significativamente la eficacia antimicrobiana en comparación con las secuencias lineales.
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