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Types of RNA01:23

Types of RNA

73.0K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.0K
Types of RNA01:20

Types of RNA

9.7K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
9.7K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.4K
Overview
80.4K
RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K

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Video Experimental Relacionado

Updated: Feb 13, 2026

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

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Ingeniería del transcriptoma con efectores CRISPR de tipo VI-D dirigidos al ARN

Silvana Konermann1, Peter Lotfy1, Nicholas J Brideau1

  • 1Laboratory of Molecular and Cell Biology, Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA; Helmsley Center for Genomic Medicine, Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA.

Cell
|March 20, 2018
PubMed
Resumen
Este resumen es generado por máquina.

Los científicos descubrieron un nuevo sistema CRISPR-Cas, tipo VI-D, y diseñaron una enzima compacta llamada CasRx. CasRx se dirige de manera eficiente al ARN en las células humanas, ofreciendo una nueva plataforma para la ingeniería del transcriptoma y posibles terapias.

Palabras clave:
CRISPR y sus derivadosEn el caso deCasRx yInterferencia del ARNOrientación al ARNempalme alternativoDemencia frontotemporalEdición de genesIngeniería genómicael tau

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CRISPR Guide RNA Cloning for Mammalian Systems

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Área de la Ciencia:

  • Microbiología
  • Biología molecular
  • La genética

Sus antecedentes:

  • Los sistemas CRISPR-Cas de clase 2 proporcionan inmunidad adaptativa en los microbios.
  • Las investigaciones anteriores se centraron en los sistemas de orientación del ADN, dejando los sistemas de orientación del ARN menos explorados.

Objetivo del estudio:

  • Identificar y caracterizar nuevos sistemas CRISPR-Cas dirigidos al ARN.
  • Para diseñar una nueva enzima dirigida al ARN para posibles aplicaciones terapéuticas.

Principales métodos:

  • Análisis bioinformático de genomas procariotas y metagenomas.
  • Caracterización bioquímica e ingeniería de proteínas de los ortólogos CRISPR-Cas identificados.
  • Ensayos funcionales in vitro e in vivo en células humanas y modelos neuronales.

Principales resultados:

  • Identificación y clasificación de un nuevo sistema CRISPR-Cas dirigido al ARN, tipo VI-D.
  • Ingeniería de una ribonucleasa compacta y altamente eficiente dirigida al ARN, CasRx, de Ruminococcus flavefaciens XPD3002.
  • Demostración de la eliminación del ARN mediada por CasRx con alta eficiencia y especificidad en células humanas.
  • Aplicación de CasRx catalíticamente inactivo para manipular el empalme alternativo y aliviar la desregulación de la tau en un modelo de demencia frontotemporal.

Conclusiones:

  • CasRx representa un nuevo módulo de unión al ARN programable para la ingeniería del transcriptoma.
  • El sistema CRISPR-Cas tipo VI-D y su efector CasRx ofrecen una plataforma versátil para la orientación del ARN.
  • CasRx es prometedor para el desarrollo terapéutico futuro, particularmente en enfermedades genéticas y neurodegenerativas.