Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

tRNA Activation02:26

tRNA Activation

23.1K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
23.1K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Acid–Base Equilibria: Activity-Based Definition of pH01:10

Acid–Base Equilibria: Activity-Based Definition of pH

1.3K
For an ideal solution, the pH is defined as the negative logarithm of the hydrogen ion concentration. For a non-ideal solution, an accurate measurement of the pH must consider the negative logarithm of the hydrogen ion activity rather than concentration. In such a solution, the pH can be more accurately defined as the negative logarithm of a product of the hydrogen ion concentration and its activity coefficient.
In solutions of very low ionic strength—for example, pure water—the...
1.3K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.7K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.7K
Activation Energy01:26

Activation Energy

87.1K
Activation energy is the minimum amount of energy necessary for a chemical reaction to move forward. The higher the activation energy, the slower the rate of the reaction. However, adding heat to the reaction will increase the rate, since it causes molecules to move faster and increase the likelihood that molecules will collide. The collision and breaking of bonds represents the uphill phase of a reaction and generates the transition state. The transition state is an unstable high-energy state...
87.1K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.9K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.9K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

STING-dependent peripheral inflammaging drives neurodegeneration via extracellular vesicles.

Cell reports·2026
Same author

Association between the use of anti-herpetic drugs and subsequent initiation of Alzheimer's disease drug treatment: Dutch population-based inception cohort study.

BMJ open·2026
Same author

Genetic Rescue of Pathogenic O-GlcNAc Dyshomeostasis Associated with Microcephaly and Motor Deficits.

eNeuro·2026
Same author

Reprogramming of bacterial virulence by lysine acetylation.

Nature communications·2026
Same author

Ubiquitination of glycogen and metabolites in cells and tissues.

Nature·2026
Same author

The Contribution of Native Protein Complexes to Targeted Protein Degradation.

ACS chemical biology·2026

Video Experimental Relacionado

Updated: Feb 12, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.0K

El perfil de la E3 ligasa basado en la actividad descubre una E3 ligasa con actividad de esterificación

Kuan-Chuan Pao1, Nicola T Wood1, Axel Knebel1

  • 1MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee, UK.

Nature
|April 13, 2018
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores descubrieron un nuevo tipo de ligasa de ubiquitina E3, MYCBP2, que modifica los residuos de treonina, no la lisina. Este hallazgo revela una nueva ubiquitinación no lisina y amplía la comprensión de la diversidad de enzimas E3 en eucariotas.

Más Videos Relacionados

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

9.4K
In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

9.8K

Videos de Experimentos Relacionados

Last Updated: Feb 12, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.0K
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

9.4K
In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

9.8K

Área de la Ciencia:

  • La bioquímica
  • Biología molecular
  • Biología celular

Sus antecedentes:

  • La ubiquitinación es una modificación post-traduccional clave que regula los procesos celulares.
  • La enzima activadora de ubiquitina (E1), la enzima conjugadora de ubiquitina (E2) y las ligasas de ubiquitina (E3) median la ubiquitinación.
  • Las ligasas E3 se clasifican en tipos RING, HECT y RBR, modificando principalmente los residuos de lisina.

Objetivo del estudio:

  • Identificar nuevas actividades de la E3 ligasa más allá de la modificación de la lisina.
  • Caracterizar el mecanismo y la especificidad del sustrato de MYCBP2 (PHR1).
  • Explorar las implicaciones de la ubiquitinación no lisina en los eucariotas superiores.

Principales métodos:

  • Perfiles de proteínas basados en la actividad de las ligasas E3 HECT y RBR.
  • Ensayos bioquímicos para determinar la especificidad del sustrato.
  • Caracterización cristalográfica de la ligasa MYCBP2 E3.

Principales resultados:

  • MYCBP2 fue identificado como una nueva ligasa E3 con actividad de esterificación.
  • MYCBP2 exhibe una selectividad intrínseca para la treonina sobre los residuos de serina.
  • La nueva clase de ligasa E3, denominada RING-Cys-relay (RCR), utiliza intermediarios de tioésteres.

Conclusiones:

  • MYCBP2 representa una clase única de E3 ligasa (RCR) con actividad de ubiquitinación sin lisina.
  • La ubiquitinación de treonina por MYCBP2 juega un papel en la regulación celular.
  • Las enzimas E3 poseen una mayor diversidad mecánica de lo que se entendía anteriormente.