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El receptor de importación nuclear inhibe la separación de fase del FUS a través de la unión a múltiples sitios

Takuya Yoshizawa¹, Rustam Ali², Jenny Jiou¹

¹Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

|April 21, 2018

Resumen

Karyopherin-β2 inhibe la separación de fase líquido-líquido (LLPS) de la proteína FUS al unirse a su PY-NLS. Esta interacción interrumpe el autoensamblaje de FUS, proporcionando información sobre la formación y regulación del condensado.

Área de la Ciencia:

La bioquímica
Biología celular
Biofísica molecular

Sus antecedentes:

La separación de fase líquido-líquido (LLPS) impulsa la formación de condensados biomoleculares sin membrana.
Los mecanismos que rigen el LLPS y la dinámica del condensado siguen siendo incompletamente entendidos.
Se sabe que la proteína de unión al ARN FUS se somete a LLPS y se localiza en condensados celulares.

Objetivo del estudio:

Investigar el papel de la cariferina-β2 en la regulación de la FUS LLPS.
Elucidar los mecanismos moleculares por los cuales la carioferina-β2 inhibe la FUS LLPS.
Explorar el potencial de la carioferina-β2 como modulador de la formación de condensados.

Principales métodos:

Pruebas de LLPS in vitro.
Estudios de las interacciones bioquímicas.
Espectroscopia de resonancia magnética nuclear (RMN).

Principales resultados:

Se encontró que la Karyopherin-β2 inhibe el FUS LLPS.
La inhibición está correlacionada con la unión de alta afinidad de la carioferina-β2 a la FUS PY-NLS.
Los análisis de RMN y bioquímicos revelaron interacciones débiles y distribuidas entre las regiones carióferina-β2 y FUS involucradas en LLPS.

Conclusiones:

Se propone un modelo en el que la unión de la cariferina-β2 a la FUS PY-NLS actúa como un andamio, permitiendo interacciones débiles que interrumpen la autoasociación de la FUS y bloquean la LLPS.
El mecanismo inhibidor de Karyopherin-β2 ofrece una estrategia potencial para controlar la formación de condensados en varios contextos celulares.

Palabras clave:

FUS y sus derivados PY-NLS (en inglés)Granulado de ARN esclerosis lateral amiotrófica Condensado biomolecular Proteína intrínsecamente desordenada Caryopherina-β2 y sus derivados Separación de la fase líquido-líquido secuencias de baja complejidad el transporte en-1

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