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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
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In a nonhomogeneous rod made up of steel and brass, restrained at both ends and subjected to a temperature change, several steps are involved in calculating the stress and compressive load. Due to the problem's static indeterminacy, one end support is disconnected, allowing the rod to experience the temperature change freely. Next, an unknown force is applied at the free end, triggering deformations in the rod's steel and brass portions. These deformations are then calculated and added...
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Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
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Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
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La histona demetilasa KDM6B regula la determinación del sexo dependiente de la temperatura en una especie de tortuga

Chutian Ge1, Jian Ye2, Ceri Weber3

  • 1Zhejiang Provincial Top Key Discipline of Biological Engineering, Zhejiang Wanli University, Ningbo, 315100, China. cge@zwu.edu.cn qiangy@zwu.edu.cn blanche.capel@duke.edu.

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La determinación del sexo dependiente de la temperatura en las tortugas está regulada por KDM6B, un factor epigenético. La interrupción de KDM6B causa la inversión de sexo de hombre a mujer, revelando un vínculo directo entre la epigenética y la determinación del sexo.

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Área de la Ciencia:

  • Biología del desarrollo
  • La epigenética
  • Herpetología

Sus antecedentes:

  • La determinación del sexo dependiente de la temperatura (TSD) es un modelo clave para la plasticidad fenotípica.
  • En las tortugas deslizantes de orejas rojas (Trachemys scripta elegans), la temperatura de incubación dicta el sexo de la descendencia.
  • Los mecanismos epigenéticos son cada vez más reconocidos como cruciales en los procesos de desarrollo.

Objetivo del estudio:

  • Investigar el papel de KDM6B en el TSD en Trachemys scripta elegans.
  • Para determinar si la expresión de KDM6B es sexualmente dimórfica y dependiente de la temperatura.
  • Para aclarar la vía genética que vincula KDM6B a la determinación del sexo.

Principales métodos:

  • Análisis de la expresión de KDM6B en embriones tempranos de T. scripta a diferentes temperaturas.
  • Realización de experimentos de derribo de KDM6B a temperaturas de determinación del macho (26 °C).
  • Investigar el efecto de KDM6B en la transcripción de Dmrt1 y la trimetilación de H3K27.
  • Evaluación del efecto de rescate de la sobreexpresión de Dmrt1.

Principales resultados:

  • KDM6B muestra expresión dimórfica sexual dependiente de la temperatura en embriones tempranos de T. scripta.
  • El knockdown de KDM6B a 26°C indujo la reversión del sexo masculino a femenino en más del 80% de los sobrevivientes.
  • KDM6B promueve directamente la transcripción del gen masculino Dmrt1 mediante la eliminación de la trimetilación H3K27.
  • La sobreexpresión de Dmrt1 rescató la reversión de sexo inducida por el knockdown de Kdm6b.

Conclusiones:

  • Este estudio establece un vínculo genético causal entre el factor epigenético KDM6B y la TSD en tortugas.
  • KDM6B actúa como un regulador clave en la vía dependiente de la temperatura para la determinación del sexo masculino.
  • Las modificaciones epigenéticas son fundamentales para comprender la plasticidad fenotípica en los reptiles.