Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

From DNA to Protein03:06

From DNA to Protein

22.5K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.5K
Mechanical Protein Functions01:58

Mechanical Protein Functions

5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
5.7K
Structural Protein Function01:56

Structural Protein Function

30.0K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.0K
Structural Protein Function01:56

Structural Protein Function

3.3K
3.3K
Mechanical Protein Function01:58

Mechanical Protein Function

2.5K
2.5K
DNA-only Transposons02:57

DNA-only Transposons

17.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Metal-Phenolic Coatings Enable Universal Design of Spherical Nucleic Acids.

Angewandte Chemie (International ed. in English)·2026
Same author

Correction to "DNA-Mediated Cellular Delivery of Functional Enzymes".

Journal of the American Chemical Society·2026
Same author

High-χ Block Copolymer Nanoreactors for the Confined Synthesis of Size-Controlled Nanoclusters.

ACS nano·2026
Same author

Programmable Stepwise Heteroepitaxial Growth of Colloidal Crystals With Different Phases.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Simplex-based model for nanoparticle grain identification in four-dimensional scanning transmission electron microscopy data.

Journal of microscopy·2026
Same author

High-entropy 1D halide perovskite piezoelectrics found by megalibrary synthesis and rapid nonlinear optical screening.

Science advances·2026
Same journal

Linker Engineering toward NIR-II Metal-Organic Framework with Maximal Emission beyond 1000 nm for Inflammatory Bowel Disease Imaging.

Journal of the American Chemical Society·2026
Same journal

Observing Kinetic Selectivity in Anthracene Photodimerization through Selective Quenching by Excited States of Proximate Rare Earth Cations.

Journal of the American Chemical Society·2026
Same journal

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026
Same journal

Large Thermo- and Mechanosalient Actuation via Cooperative Twist Elasticity-Induced Packing Motif Conversion.

Journal of the American Chemical Society·2026
Same journal

Discovery and Biosynthesis of Lanthipeptides Featuring an Azepinoindole Scaffold by Radical <i>S</i>-Adenosylmethionine Enzyme-Catalyzed C-C Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Enantiopurity-Controlled Magnetism in a Two-Dimensional Organic-Inorganic Material.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: Feb 10, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

11.0K

Proteínas bivalentes funcionalizadas por el ADN

Janet R McMillan1, Chad A Mirkin1

  • 1Department of Chemistry and International Institute for Nanotechnology , Northwestern University , 2145 Sheridan Road , Evanston , Illinois 60208 , United States.

Journal of the American Chemical Society
|May 26, 2018
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores crearon nuevos conjugados de ADN y proteínas que se autoensamblan en materiales proteicos unidimensionales. Este ensamblaje mediado por ADN forma superestructuras periódicas de tipo de alambre con enlaces direccionales.

Más Videos Relacionados

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.7K
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

14.2K

Videos de Experimentos Relacionados

Last Updated: Feb 10, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

11.0K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.7K
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

14.2K

Área de la Ciencia:

  • Bioconjugación Química
  • Ciencias de los materiales
  • Nanotecnología

Sus antecedentes:

  • Las proteínas pueden ser funcionalizadas con ADN para crear materiales de autoensamblaje.
  • Lograr el control direccional en el ensamblaje de proteínas es un desafío con los métodos actuales.

Objetivo del estudio:

  • Para sintetizar y caracterizar los conjugados de ADN bivalente de la beta-galactosidasa (βGal).
  • Para demostrar el acceso programable a los materiales de proteínas unidimensionales utilizando enlaces direccionales de ADN.
  • Para diseñar nuevas clases de superestructuras a través de interacciones controladas de proteínas.

Principales métodos:

  • Síntesis de la proteína βGal funcionalizada con pares de oligonucleótidos.
  • Caracterización mediante electroforesis en gel, microscopía electrónica de crio-transmisión y microscopía electrónica de transmisión de manchas negativas.
  • Experimentos de fusión para analizar la estabilidad duplex del ADN y los modos de unión.

Principales resultados:

  • Se han sintetizado con éxito conjugados bivalentes de ADN y proteína.
  • Demostró la formación de superestructuras de proteínas periódicas de tipo de alambre a través de la hibridación del ADN.
  • Se observaron transiciones de fusión estrechas y elevadas, lo que confirma la unión mediada por el ADN y las interacciones diseñadas.
  • Mostró enlaces direccionales de ADN con sólo dos modificaciones de ADN.

Conclusiones:

  • La unión direccional del ADN se puede lograr con modificaciones mínimas del ADN en las proteínas.
  • Este enfoque permite la ingeniería de materiales proteicos unidimensionales programables.
  • Se pueden realizar nuevas superestructuras con interacciones controladas, avanzando en tecnologías de autoensamblaje.