Video Experimental Relacionado

Updated: Feb 9, 2026

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Diversidad y regulación del tipo de célula cnidaria revelada por la secuencia de ARN unicelular de todo el organismo

Arnau Sebé-Pedrós¹, Baptiste Saudemont², Elad Chomsky¹

¹Department of Computer Science and Applied Mathematics and Department of Biological Regulation, Weizmann Institute of Science, 76100 Rehovot, Israel.

|June 2, 2018

Resumen

Este estudio mapea los tipos de células en Nematostella vectensis utilizando transcriptómica de una sola célula, revelando ocho amplias clases de células y diversos subtipos de neuronas. Identifica los códigos reguladores de genes que impulsan la expresión específica de la célula, ofreciendo información sobre la evolución animal.

Área de la Ciencia:

Biología del desarrollo evolutivo
La genómica
Biología celular

Sus antecedentes:

La diversificación del tipo de célula es crucial para la evolución animal.
Los estudios anteriores carecían de un análisis integral de la regulación génica en diversos tipos y especies de células.

Objetivo del estudio:

Mapear sistemáticamente los tipos de células y los programas reguladores de genes en el cnidario Nematostella vectensis.
Comprender la evolución de la regulación génica específica de las células en animales no bilaterales.

Principales métodos:

Transcriptómica de una sola célula de todo el organismo.
Perfiles de cromatina y análisis de la secuencia de motivos.
Análisis de la expresión del factor de transcripción.

Principales resultados:

Identificó ocho grandes clases de células en Nematostella, incluidos diversos subtipos de neuronas.
Se caracteriza por la diversificación específica del linaje de las neuronas.
Códigos reguladores descubiertos que rigen la expresión génica específica de la célula.

Conclusiones:

Nematostella exhibe una complejidad significativa del tipo de célula.
Proporciona información sobre la evolución de la regulación genómica específica de las células animales.
Destaca la importancia de los programas reguladores de genes en la evolución del tipo de célula.

Palabras clave:

ATAC-seq (en inglés)Evolución del tipo celular expresión génica regulación del genoma Multicelularidad el neurón genómica de una sola célula Factores de transcripción

Más Videos Relacionados

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Videos de Conceptos Relacionados

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Cell Diversity

Cell Diversity

The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular...

Types of Genetic Transfer Between Organisms

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.

pH Regulation in Cells

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...