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Papel de la proteína nucleocápsida en la regulación de la síntesis de ARN del virus de la estomatitis vesicular
Cell
|May 1, 1985
Resumen
Dos anticuerpos dirigidos al virus de la estomatitis vesicular (VSV) nucleocapsid proteína N revelan su papel crítico en la replicación viral. La actividad de anticuerpos demuestra que la disponibilidad de proteína N controla la replicación del genoma VSV.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Inmunología Inmunología.
Sus antecedentes:
- La replicación del virus de la estomatitis vesicular (VSV) se basa en su complejo de proteínas nucleocapsid (N) y polimerasa.
- No se comprende completamente el papel preciso de la proteína N libre frente a la proteína N unida al nucleocáptido en la replicación de VSV.
- Los factores del huésped y la fosforilación de la proteína NS también están implicados en la síntesis de ARN genómico VSV.
Objetivo del estudio:
- Investigar las distintas funciones de la proteína nucleocapsida (N) del virus de la estomatitis vesicular (VSV) en la replicación viral utilizando anticuerpos monoclonales.
- Para dilucidar el impacto de la disponibilidad de proteína N en la síntesis del genoma de VSV in vivo e in vitro.
Principales métodos:
- Se utilizaron dos anticuerpos monoclonales con diferentes características de unión a la proteína VSV N.
- Evaluación de los efectos de los anticuerpos en la transcripción y replicación del VSV en células microinyectadas.
- Impacto del anticuerpo analizado en la síntesis de ARN genómico in vitro.
Principales resultados:
- Anticorpo 1, que se une a la proteína N y a los nucleocápsidos, inhibe la transcripción y protege a las células contra el VSV.
- El anticuerpo 2, que se une selectivamente a la proteína N libre, retrasa la aparición del virus progenitor sin inhibir la transcripción.
- Tanto los resultados in vivo como in vitro indicaron que la disponibilidad de proteínas N controla la replicación del genoma VSV.
Conclusiones:
- La disponibilidad de la proteína nucleocápside (N) del virus de la estomatitis vesicular (VSV) es un regulador clave de la replicación del genoma de VSV.
- Esta regulación ocurre independientemente del acceso de la polimerasa a los factores huésped y de la fosforilación de la proteína NS.
- Los anticuerpos monoclonales dirigidos a la proteína N proporcionan herramientas valiosas para diseccionar los mecanismos de replicación del VSV.
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