Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

40.9K
Overview
40.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

15.7K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
15.7K
Overview of DNA Repair02:25

Overview of DNA Repair

33.8K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.8K
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

48.7K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
48.7K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

53.1K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
53.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Fluorescence Anisotropy Applied to Measure Aptamer-Protein Interactions.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Anticodon loop remodeling and D-stem shape drive the specific recognition of ANN-decoding tRNAs for t6A modification.

Nucleic acids research·2026
Same author

Tunable gene expression in zebrafish using RiboSCALE.

bioRxiv : the preprint server for biology·2026
Same author

Publisher Correction: Unlocking chemical diversity in aptamers with DNA orthogonal barcodes.

Nature chemistry·2026
Same author

Unlocking chemical diversity in aptamers with DNA orthogonal barcodes.

Nature chemistry·2026
Same author

UVA Irradiation Promotes ROS-Mediated Formation of the Common Deletion in Mitochondrial DNA.

Life (Basel, Switzerland)·2026

Video Experimental Relacionado

Updated: Feb 8, 2026

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
10:43

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Published on: June 3, 2017

11.7K

Mapeo de todo el genoma de daño oxidativo del ADN por clic-código-seq

Junzhou Wu1, Maureen McKeague1, Shana J Sturla1

  • 1Department of Health Sciences and Technology , ETH Zürich , Schmelzbergstrasse 9 , 8092 Zürich , Switzerland.

Journal of the American Chemical Society
|June 27, 2018
PubMed
Resumen

Los científicos desarrollaron Click-code-seq para mapear el daño oxidativo del ADN a una resolución de un solo nucleótido en todo el genoma. Este nuevo método revela patrones de oxidación distintos vinculados a los procesos celulares, superando las limitaciones de secuenciación anteriores.

Más Videos Relacionados

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.6K
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

59.4K

Videos de Experimentos Relacionados

Last Updated: Feb 8, 2026

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
10:43

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Published on: June 3, 2017

11.7K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.6K
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

59.4K

Área de la Ciencia:

  • La genómica
  • Biología molecular
  • La bioquímica

Sus antecedentes:

  • El mapeo preciso del daño del ADN a resolución de un solo nucleótido es crucial para comprender su impacto biológico.
  • Los métodos actuales luchan con la baja abundancia y la amplificación del daño del ADN, lo que dificulta el análisis de todo el genoma.
  • Mapear el daño oxidativo del ADN, particularmente la oxidación de la guanina, en alta resolución sigue siendo un desafío significativo.

Objetivo del estudio:

  • Desarrollar un método de secuenciación de alto rendimiento y resolución de un solo nucleótido del daño oxidativo del ADN en todo el genoma.
  • Para superar las limitaciones de las técnicas existentes en la detección y cartografía de los adductos de ADN.

Principales métodos:

  • Combinó la especificidad de las enzimas de reparación de ADN con la ligadura de ADN de clic para insertar un código de localización.
  • Se habilitado la secuenciación de alto rendimiento del daño oxidativo del ADN en la resolución de nucleótidos dentro de un genoma.

Principales resultados:

  • Identificó miles de sitios de daño oxidativo en el genoma.
  • Se observaron patrones distintos de oxidación correlacionados con la transcripción, la estructura de la cromatina y el potencial de oxidación química.
  • Demostró la capacidad de Click-code-seq para generar datos de modificación completos y específicos de la secuencia.

Conclusiones:

  • Click-code-seq supera efectivamente las barreras anteriores en la secuenciación de daños en el ADN.
  • Este enfoque proporciona una forma novedosa de generar información detallada y específica de la secuencia sobre las modificaciones químicas en genomas enteros.
  • Facilita una comprensión más profunda de la relación entre el daño del ADN, su ubicación y las consecuencias biológicas.