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Updated: Jun 20, 2026
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Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
La proteína quinasa C fosforila pp60src en un nuevo sitio
Cell
|October 1, 1985
Resumen
La proteína quinasa C fosforila las pp60src en la serina 12 in vivo. Esta modificación, inducida por promotores tumorales y diacilglicerol, fue confirmada utilizando proteína quinasa C purificada in vitro, destacando un nuevo evento de fosforilación.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La señalización celular.
- Oncología Oncología Oncología.
Sus antecedentes:
- pp60v-src (proteína transformadora del virus del sarcoma crudo) y pp60c-src (homólogo celular) son proteínas clave en el crecimiento celular.
- La fosforilación es un mecanismo regulador crítico para la función de las proteínas.
Objetivo del estudio:
- Para identificar la quinasa responsable de la fosforilación in vivo de pp60src en la serina 12.
- Para investigar el papel de la proteína quinasa C en la modificación de pp60src.
Principales métodos:
- En estudios de fosforilación in vivo se utilizaron promotores tumorales (12-O-tetradecanoilforbol-13-acetato, teleocidina) y diacilglicerol.
- Ensayos de kinasa in vitro con pp60c-src/pp60v-src purificado y varias proteínas quinasas específicas de serina/treonina.
- Fosforilación in vitro de un péptido sintético que imita el extremo N de pp60c-src utilizando proteína quinasa C. purificada.
Principales resultados:
- Los promotores tumorales y el diacilglicerol indujeron una fosforilación significativa de pp60src en la serina 12 in vivo.
- Sólo la proteína quinasa C purificada fosforila pp60c-src y pp60v-src en la serina 12 in vitro.
- La proteína quinasa C purificada fosforila un péptido sintético en la serina 12, confirmando su sitio de actividad.
Conclusiones:
- La proteína quinasa C es la principal quinasa responsable de la fosforilación de pp60src en la serina 12 in vivo.
- Este nuevo evento de fosforilación por la proteína quinasa C puede tener implicaciones fisiológicas significativas.
- Se necesita más investigación para dilucidar las consecuencias funcionales de la fosforilación de la serina 12.
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