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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Published on: March 7, 2019
La levadura de fisión Schizosaccharomyces pombe extirpa correctamente una secuencia de secuencia intermedia de
Nature
|November 7, 1985
Resumen
La levadura de fisión, Schizosaccharomyces pombe, elimina con precisión las secuencias intermedias de los genes eucariotas superiores. Este hallazgo sugiere que Schizosaccharomyces pombe es un modelo superior para estudiar el empalme de ARN y la expresión de genes eucariotas en comparación con Saccharomyces cerevisiae.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- Biología de la levadura Biología de la levadura.
Sus antecedentes:
- La levadura en ciernes (Saccharomyces cerevisiae) lucha con la expresión génica heteróloga debido a la eliminación ineficiente de las secuencias intermedias de las transcripciones eucarióticas superiores.
- Esta ineficiencia en S. cerevisiae está relacionada con un estricto requisito de una caja TACTAAC cerca del extremo 3' de las secuencias intermedias para el empalme.
- La levadura de fisión (Schizosaccharomyces pombe) posee intrones con secuencias conservadas similares a las de los eucariotas superiores, lo que sugiere un potencial para mejorar las capacidades de empalme.
Objetivo del estudio:
- Investigar la capacidad de Schizosaccharomyces pombe para extirpar con precisión secuencias intermedias de transcripciones de genes eucariotas superiores.
- Evaluar Schizosaccharomyces pombe como un sistema modelo para estudios de empalme de ARN y expresión génica heteróloga.
Principales métodos:
- Clonado del ADN del antígeno T pequeño del virus simio 40 (SV40).
- Expresión del ADN viral clonado en las células de Schizosaccharomyces pombe.
- Análisis del procesamiento de transcripción para evaluar la eliminación de secuencias intermedias y la utilización del sitio de empalme.
Principales resultados:
- Schizosaccharomyces pombe utiliza con precisión los sitios de empalme 5' y 3' del transcrito del antígeno T pequeño del virus simio 40 (SV40).
- La levadura de fisión demuestra una excisión efectiva de las secuencias intermedias de la transcripción del gen eucariótico superior probado.
Conclusiones:
- Schizosaccharomyces pombe demuestra un empalme preciso del ARN de las transcripciones eucarióticas superiores, a diferencia de Saccharomyces cerevisiae.
- Schizosaccharomyces pombe presenta un organismo modelo más adecuado para el estudio genético del empalme de ARN.
- Esta especie de levadura ofrece un sistema prometedor para la expresión de genes eucariotas superiores.
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