Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

807
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
807
Viral Structure00:56

Viral Structure

74.7K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.7K
Genomics02:02

Genomics

40.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.8K
Viral Recombination00:57

Viral Recombination

25.2K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shredding.

Nature·2026
Same author

Toward a Random Background for Ligand Optimization.

bioRxiv : the preprint server for biology·2026
Same author

Selective Elimination of TP53 Mutant Cells by Transcript-Activated Chromatin Shredding.

bioRxiv : the preprint server for biology·2026
Same author

Two decades of PARP inhibitor synthetic lethality in cancer.

Nature·2026
Same author

Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.

Proceedings of the Japan Academy. Series B, Physical and biological sciences·2026
Same author

Correction to: Elevated APOBEC3B expression drives a kataegic-like mutation signature and replication stress-related therapeutic vulnerabilities in p53-defective cells.

British journal of cancer·2026

Video Experimental Relacionado

Updated: Feb 7, 2026

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
12:26

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

10.1K

Reprogramación de la función y la especificidad de las células T humanas con orientación al genoma no viral

Theodore L Roth1,2,3,4,5, Cristina Puig-Saus6, Ruby Yu3,4,5

  • 1Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA.

Nature
|July 12, 2018
PubMed
Resumen

Este estudio introduce un sistema de edición del genoma CRISPR-Cas9 no viral para la reprogramación eficiente de las células T. Este método permite inserciones precisas de ADN de gran tamaño para aplicaciones terapéuticas, incluida la corrección de enfermedades autoinmunes y la inmunoterapia contra el cáncer.

Más Videos Relacionados

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

958
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

15.0K

Videos de Experimentos Relacionados

Last Updated: Feb 7, 2026

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
12:26

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

10.1K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

958
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

15.0K

Área de la Ciencia:

  • Inmunología
  • Biología molecular
  • Terapia génica

Sus antecedentes:

  • La reprogramación tradicional de células T se basa en vectores virales, que son costosos y requieren mucho tiempo.
  • Los vectores virales carecen de una integración genómica precisa, lo que plantea desafíos para las aplicaciones terapéuticas.
  • La edición del genoma ofrece la inserción de genes dirigidos, pero se ha enfrentado a limitaciones con grandes secuencias de ADN.

Objetivo del estudio:

  • Desarrollar un sistema CRISPR-Cas9 no viral para la inserción eficiente y específica de grandes secuencias de ADN en células T humanas primarias.
  • Demostrar el potencial terapéutico de este sistema en la corrección de defectos genéticos y la ingeniería de células T para la inmunoterapia del cáncer.

Principales métodos:

  • Desarrolló un sistema de orientación del genoma CRISPR-Cas9 para la inserción mediada por la reparación no viral y dirigida por la homología de secuencias de ADN grandes (> 1 kb).
  • Aplicó el sistema a las células T humanas primarias, evaluando la viabilidad celular, la función y la integración genómica precisa.
  • Utilizó el sistema para corregir una mutación IL2RA en modelos de enfermedades autoinmunes e ingeniería de células T con un receptor de células T dirigido al cáncer (TCR).

Principales resultados:

  • Se logró la inserción rápida y eficiente de grandes secuencias de ADN en células T humanas primarias sin vectores virales, preservando la viabilidad y la función de las células.
  • Se corrigió con éxito una mutación patógena de IL2RA, restaurando la función de señalización en las células T de pacientes con enfermedad autoinmune monogénica.
  • Células T diseñadas con un nuevo TCR que reconocen específicamente antígenos tumorales, demostrando respuestas anti-tumorales efectivas in vitro e in vivo.

Conclusiones:

  • El sistema de orientación del genoma CRISPR-Cas9 no viral permite la ingeniería genética rápida y flexible de las células inmunes humanas primarias.
  • Esta tecnología tiene una promesa preclínica significativa para el desarrollo de nuevas terapias basadas en células para enfermedades autoinmunes y cáncer.
  • La orientación del genoma no viral ofrece una alternativa más eficiente y potencialmente rentable a los vectores virales para terapias basadas en células T.