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Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
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La fusión de gametos desencadena el ensamblaje bipartito del factor de transcripción para bloquear la
Aleksandar Vještica1, Laura Merlini1, Pedro Junior Nkosi1
1Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
Nature
|August 10, 2018
Resumen
La reproducción sexual se basa en el bloqueo de la re-fertilización después de la fusión inicial. Un factor de transcripción bipartito, formado por el péptido Mi de las células M y la proteína Pi de las células P, bloquea rápidamente la refertilización e induce la meiosis en Schizosaccharomyces pombe.
Área de la Ciencia:
- Biología molecular
- La genética
- Micología
Sus antecedentes:
- La reproducción sexual requiere un control preciso de la ploidia y la formación de cigotos.
- El modelo fúngico Schizosaccharomyces pombe fusiona células haploides para formar un cigoto diploide que se somete a meiosis.
Objetivo del estudio:
- Investigar los mecanismos que impiden la re-fertilización después de la fusión inicial de gametos en Schizosaccharomyces pombe.
- Para aclarar cómo se establece la expresión génica asimétrica después de la fusión.
- Comprender el papel de un factor de transcripción bipartito en la regulación de la reproducción sexual.
Principales métodos:
- Identificación y análisis de mutantes que muestran fusión celular transitoria.
- Investigando la expresión génica asimétrica después del intercambio del citosol.
- Reconstrucción del factor de transcripción bipartito utilizando interactores sintéticos.
Principales resultados:
- La fusión celular transitoria conduce a destinos celulares distintos: meiosis haploide letal en el compañero P y persistencia en la célula M.
- La transcripción zigótica asimétrica se inicia rápidamente en el núcleo P, dependiente del complejo Mi-Pi.
- La transcripción zigótica retrasada da lugar a una progenie poliploide/aneuploide debido a la re-fertilización.
Conclusiones:
- Una conexión citoplasmática en la fusión de gametos permite una reconstitución rápida y asimétrica de un factor de transcripción bipartito.
- Este mecanismo bloquea efectivamente la re-fertilización e inicia la meiosis, asegurando la estabilidad del genoma durante la reproducción sexual.
- Los hallazgos proporcionan información sobre los procesos fundamentales de reproducción sexual y mantenimiento del genoma.
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