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Recorrido del plegamiento de la actina dirigido por la TRiC de la chaperonina eucariota
David Balchin1, Goran Miličić1, Mike Strauss2
1Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Cell
|August 14, 2018
Resumen
La chaperonina eucariota TRiC (complejo de anillos TCP-1) facilita el plegamiento de la actina, a diferencia de las GroEL/GroES bacterianas. TRiC se une a un intermediario dinámico de actina, promoviendo su conformación nativa a través de cambios conformacionales dependientes de ATP.
Área de la Ciencia:
- Biología molecular
- La bioquímica
- Biología estructural
Sus antecedentes:
- La chaperonina TRiC (complejo de anillos TCP-1) es esencial para el plegamiento de las proteínas eucariotas del citoesqueleto como la actina.
- Las chaperoninas bacterianas (GroEL/GroES) son ineficaces para doblar la actina, lo que pone de relieve las diferencias en los sistemas de chaperoninas.
Objetivo del estudio:
- Aclarar el mecanismo por el cual TRiC facilita la progresión conformacional de la actina a su estado nativo.
- Para comparar los mecanismos de plegado de TRiC y GroEL/GroES utilizando la actina como sustrato.
Principales métodos:
- Técnicas espectroscópicas
- Análisis estructural
- Ensayos bioquímicos
Principales resultados:
- La actina pobla un estado dinámico cinéticamente atrapado que resiste el plegamiento espontáneo.
- TRiC se une a este intermediario de actina, estabilizando una conformación extendida con una estructura secundaria similar a la nativa.
- GroEL/GroES estabiliza la actina en su estado desplegado.
- La unión de ATP a TRiC induce cambios conformacionales asimétricos, lo que lleva a la liberación parcial de actina y al plegamiento posterior tras la liberación completa mediada por la hidrólisis de ATP.
Conclusiones:
- Las características estructurales y funcionales únicas de TRiC son cruciales para dirigir el plegamiento de su sustrato eucariota obligatorio, la actina.
- El mecanismo implica la unión específica de un intermediario de plegado y la dinámica conformacional impulsada por ATP para facilitar el plegado productivo.
Palabras clave:
El CCTFCS y sus derivadosGroEL (en inglés)Los Estados miembrosH/DXEl TRiCLa actinaEl chaperónMicroscopía criolectrónicaEn el caso de las empresas de transporte de mercancíasEspectroscopia de correlación cruzada de fluorescencia de doble colorEspectroscopia de correlación por fluorescenciaIntercambio de hidrógeno y deuteriotransferencia de electrones inducida por la luz (PET) - FCSMás Videos Relacionados
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